NADH plays the vital role for chiral pure D-(−)-2,3-butanediol production in Bacillus subtilis under limited oxygen conditions

• Published in: Biotechnology and bioengineering Vol. 111; no. 10; pp. 2126 - 2131
• Main Authors: Fu, Jing, Wang, Zhiwen, Chen, Tao, Liu, Weixi, Shi, Ting, Wang, Guanglu, Tang, Ya-jie, Zhao, Xueming
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.10.2014; Wiley Subscription Services, Inc
• Subjects: 3-butanediol; acetoin reductase; Availability; Bacillus subtilis; Bacillus subtilis - enzymology; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacteria; Biotechnology; Butylene Glycols - chemistry; Butylene Glycols - metabolism; cofactor engineering; Comparative analysis; D-(−)-2; Dissolved oxygen; D‐(−)‐2,3‐butanediol; Industrial Microbiology; Level (quantity); Metabolic Engineering; Microorganisms; NAD - metabolism; NADH; NADP Transhydrogenases - genetics; NADP Transhydrogenases - metabolism; Oxygen; Oxygen - metabolism; Pools; Purity; Reductases; Stereoisomerism; Strategy; Substrates; UdhA; UdhA; metabolic engineering; acetoin reductase; Bacillus subtilis; D-(−)-2,3-butanediol; cofactor engineering
• ISSN: 0006-3592; 1097-0290; 1097-0290
• DOI: 10.1002/bit.25265

ABSTRACT Compared with traditional pathogenic producers, Bacillus subtilis as a Class I microorganism offers many advantages for industrial‐scale 2,3‐butanediol production. Unlike previous reports in which two stereoisomers (with a ratio of 3:2) were produced, we first found that wild type B. subtil...