The use of THP-1 cells as a model for mimicking the function and regulation of monocytes and macrophages in the vasculature

• Published in: Atherosclerosis Vol. 221; no. 1; pp. 2 - 11
• Main Author: Qin, Zhenyu
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ireland Ltd 01.03.2012; Elsevier
• Subjects: adipocytes; Adipocytes - pathology; adipose tissue; atherogenesis; atherosclerosis; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Blood Platelets; Blood Platelets - pathology; Blood Vessels; Blood Vessels - metabolism; Blood Vessels - pathology; Blood vessels and receptors; Cardiology. Vascular system; Cardiovascular; Cell Communication; Cell Line, Tumor; diabetes mellitus; endothelial cells; Endothelial Cells - pathology; Epigenesis, Genetic; Epigenetics; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; genetics; glucose; histones; Humans; inflammation; leukemia; Leukemia, Monocytic, Acute; Leukemia, Monocytic, Acute - genetics; Leukemia, Monocytic, Acute - metabolism; Leukemia, Monocytic, Acute - pathology; lipopolysaccharides; low density lipoprotein; Macrophage; macrophages; Macrophages - metabolism; Macrophages - pathology; Medical sciences; metabolism; microRNA; MicroRNAs; MicroRNAs - metabolism; Monocyte; monocytes; Monocytes - metabolism; Monocytes - pathology; myocytes; Myocytes, Smooth Muscle; Myocytes, Smooth Muscle - pathology; obesity; pathology; pathophysiology; patients; physiology; smooth muscle; T-lymphocytes; T-Lymphocytes - pathology; Transcriptome; Vascular Diseases; Vascular Diseases - genetics; Vascular Diseases - metabolism; Vascular Diseases - pathology; Vertebrates: cardiovascular system; CE; TPA; Monocyte; PMA; Transcriptome; CM; siRNA; 1α,25(OH)2D3; PBMC; LPS; pTHP-1 cells; TG; LDL; HAEC; PDGF; Epigenetics; HUVEC; miRNA; microRNA; VSMC; Macrophage; triglyceride; low density lipoprotein; 1α, 25-dihydroxyvitamin D 3; 1α,25(OH) 2D 3; chylomicron; platelet-derived growth factor; PMA treated THP-1 cells; human aortic endothelial cell; small interfering RNA; phorbol-12-myristate-13-acetate; 12-O-tetradecanoylphorbol-13-acetate; human umbilical vein endothelial cell; vascular smooth muscle cell; lipopolysaccharide; cholesteryl ester; peripheral blood mononuclear cell; Vascular disease; Atherosclerosis; Cardiovascular disease; Differential diagnostic

Since their establishment thirty years ago, THP-1 cells have become one of most widely used cell lines to investigate the function and regulation of monocytes and macrophages in the cardiovascular system. However, because this cell line was derived from the blood of a patient with acute monocytic le...