Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells

• Published in: PLoS genetics Vol. 16; no. 10; p. e1009041
• Main Authors: van Ravesteyn, Thomas W., Arranz Dols, Marcos, Pieters, Wietske, Dekker, Marleen, te Riele, Hein
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.10.2020; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Biology; Biology and life sciences; Cancer; Cell Line; Cells; Control; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA Mismatch Repair - genetics; DNA repair; DNA replication; DNA Replication - genetics; DNA, Single-Stranded - genetics; Efficiency; Gene Editing; Gene Targeting; Genetic aspects; Genome editing; Genomes; Homology; Humans; Hybridization; Immunology; Mammalian cells; Mammals - genetics; Mismatch repair; Mutation; Mutation - genetics; Oligonucleotides; Oligonucleotides - genetics; Physical sciences; Replication; Research and Analysis Methods; Single-stranded DNA; Stem cells; Transfection; Netherlands

Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex is prone to detection by DNA mismatch repair...