A Glucose Sensor Fabricated by Piezoelectric Inkjet Printing of Conducting Polymers and Bienzymes

• Published in: Analytical Sciences Vol. 27; no. 4; p. 375
• Main Authors: YUN, Yeon Hee, LEE, Byung Kook, CHOI, Ji Suk, KIM, Sungwon, YOO, Bongyoung, KIM, Yong Shin, PARK, Kinam, CHO, Yong Woo
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2011; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Analytical Chemistry; Biosensing Techniques - methods; Chemistry; Electric Conductivity; Electrochemistry; Glucose - analysis; Glucose Dehydrogenases - metabolism; Horseradish Peroxidase - metabolism; Ink; Polystyrenes - chemistry; Printing - methods; Thiophenes - chemistry; Tin Compounds - chemistry
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.27.375

Piezoelectric inkjet printing of polymers and proteins holds great promise for fabrication of miniaturized bioelectronic devices, such as biochips and biosensors. In this study, a bienzymatic glucose biosensor prototype based on poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) (PEDOT-PSS), glucose oxidase (GOD), and horseradish peroxidase (HRP) was fabricated by a piezoelectric inkjet printer. An aqueous bioelectrical ink containing PEDOT-PSS, GOD, and HRP was prepared and printed on an indium-tin-oxide (ITO)-coated poly(ethylene terephthalate) (PET) film. The PEDOT-PSS/GOD/HRP sensor was covered with a cellulose acetate membrane. The use of bienzymatic sensing combined with conducting polymers via piezoelectric inkjet printing showed a synergistic effect resulting in significant amplification of the response signal. The glucose sensor reached steady-state current density within 3 s, indicating a fast response time, and exhibited a linear dose-dependent electrochemical response with high sensitivity. The overall result demonstrates that a glucose sensor with high sensitivity could be readily fabricated by a piezoelectric inkjet printing system.