Molecular, serological and in vitro culture-based characterization of Bourbon virus, a newly described human pathogen of the genus Thogotovirus

• Published in: Journal of clinical virology Vol. 73; pp. 127 - 132
• Main Authors: Lambert, Amy J., Velez, Jason O., Brault, Aaron C., Calvert, Amanda E., Bell-Sakyi, Lesley, Bosco-Lauth, Angela M., Staples, J. Erin, Kosoy, Olga I.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2015
• Subjects: Allergy and Immunology; Animals; Bourbon virus; Cell Line; Characterization; Chlorocebus aethiops; Disease Models, Animal; Genome, Viral; HeLa Cells; Humans; In vitro growth; Infectious Disease; Influenza, Human - immunology; Influenza, Human - virology; Ixodidae; Male; Mice; Molecular; Orthomyxovirus; Phylogeny; Serological; Thogotovirus; Thogotovirus - classification; Thogotovirus - genetics; Thogotovirus - isolation & purification; Vero Cells; Viral Load; ORF; Bourbon virus; HTS; MOI; IP; Molecular; PRNT; Characterization; In vitro growth; CDS; RT-PCR; Serological; IC; BRBV; NJ; DPI; Thogotovirus; PFU; open reading frame; intracranial; days post onset; multiplicity of infection; Centers for Disease Control and Prevention; plaque reduction neutralization test; high throughput sequencing; neighbor joining; intraperitoneal; plaque forming unit; reverse transcription polymerase chain reaction

•Full-genomic analyses indicate that BRBV is a distinct member of the genus Thogotovirus.•Serological characterization further indicates that BRBV is a distinct member of the genus Thogotovirus.•Growth of BRBV in cell culture suggests association with tick and mammalian hosts.•Preliminary evaluation...