Improved Boronate Affinity Electrophoresis by Optimization of the Running Buffer for a Single-step Separation of piRNA from Mouse Testis Total RNA

• Published in: Analytical Sciences Vol. 34; no. 5; pp. 627 - 630
• Main Authors: SATO, Yusuke, IWASAWA, Daijiro, HUI, Kuo Ping, NAKAGOMI, Rena, NISHIZAWA, Seiichi
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2018; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: 2′-O-methylatyion; Affinity; Analytical Chemistry; Boronate affinity electrophoresis; Buffers; Chemistry; Electrophoresis; Optimization; phenylboronic acid; piRNA; Ribonucleic acid; Ribose; RNA; Separation; 2; methylatyion; phenylboronic acid; Boronate affinity electrophoresis; separation; piRNA; 2′-O-methylatyion

Here we examined optimization of the running buffer in boronate affinity electrophoresis for improved separation of PIWI-interacting RNA (piRNA) with 2′-O-methylated ribose in 3′-terminal nucleotide. The use of Good’s buffer, such as HEPES, significantly increased the separation efficiency for piRNA...