The thrombogram: monitoring thrombin generation in platelet-rich plasma

A method is described in which thrombin activity in clotting plasma can be monitored through the continuous measurement of the fluorescent split-product of the substrate Z-Gly-Gly-Arg-AMC. The signal is not impaired by turbidity; therefore proper measurement is not disturbed by the occurrence of a c...

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Bibliographic Details
Published inThrombosis and haemostasis Vol. 83; no. 4; p. 589
Main Authors Hemker, H C, Giesen, P L, Ramjee, M, Wagenvoord, R, Béguin, S
Format Journal Article
LanguageEnglish
Published Germany 01.04.2000
Subjects
Area Under Curve
Blood Coagulation Tests
Coumarins - analysis
Fluorescent Dyes - analysis
Fluorometry - instrumentation
Fluorometry - methods
Humans
Oligopeptides - analysis
Plasma
Platelet Count
Thrombin - analysis
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Summary:A method is described in which thrombin activity in clotting plasma can be monitored through the continuous measurement of the fluorescent split-product of the substrate Z-Gly-Gly-Arg-AMC. The signal is not impaired by turbidity; therefore proper measurement is not disturbed by the occurrence of a clot or the presence of platelets and direct measurement in platelet rich plasma is possible.
ISSN:0340-6245
DOI:10.1055/s-0037-1613868