Knockout of STAT3 in skeletal muscle does not prevent high-fat diet-induced insulin resistance

• Published in: Molecular metabolism (Germany) Vol. 4; no. 8; pp. 569 - 575
• Main Authors: White, Amanda T, LaBarge, Samuel A, McCurdy, Carrie E, Schenk, Simon
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.08.2015; Elsevier
• Subjects: Brief Communication; Clamp; Cre-LoxP; Endocrinology & Metabolism; Glucose homeostasis; In vivo; Obesity; STAT3; adhesion G protein-coupled receptor E1; Glucose homeostasis; In vivo; Adgre1; HFD; IS-GDR; EDL; hyperinsulinemic-euglycemic; muscle creatine kinase; mKO; insulin-stimulated glucose disposal rate; muscle-specific knockout of STAT3; GA; normal chow, control diet; interleukin; WT; Cre-LoxP; knockout; Obesity; CON; IL; KO; 2-deoxyglucose; STAT3; T2D; adipose tissue; glucose infusion rate; signal transducer and activator of transcription 3; HGP; GIR; hepatic glucose production; AT; type 2 diabetes; MCK; HYP-EUG; high-fat diet; extensor digitorum longus; Clamp; gastrocnemius; wild-type; 2DOG; WT, wild-type; mKO, muscle-specific knockout of STAT3; 2DOG, 2-deoxyglucose; STAT3, signal transducer and activator of transcription 3; HGP, hepatic glucose production; EDL, extensor digitorum longus; T2D, type 2 diabetes; GA, gastrocnemius; Adgre1, adhesion G protein-coupled receptor E1; IS-GDR, insulin-stimulated glucose disposal rate; KO, knockout; MCK, muscle creatine kinase; HFD, high-fat diet; IL, interleukin; CON, normal chow, control diet; AT, adipose tissue; GIR, glucose infusion rate; HYP-EUG, hyperinsulinemic-euglycemic

Abstract Objective Increased signal transducer and activator of transcription 3 (STAT3) signaling has been implicated in the development of skeletal muscle insulin resistance, though its contribution, in vivo , remains to be fully defined. Therefore, the aim of this study was to determine whether kn...