The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin

We identified the d‐galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome‐wide expression anal...

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Published inFEBS letters Vol. 590; no. 12; pp. 1804 - 1815
Main Authors Alazi, Ebru, Niu, Jing, Kowalczyk, Joanna E., Peng, Mao, Aguilar Pontes, Maria Victoria, Kan, Jan A. L., Visser, Jaap, Vries, Ronald P., Ram, Arthur F. J.
Format Journal Article
LanguageEnglish
Published England Federation of European Biochemical Societies 01.06.2016
John Wiley and Sons Inc
Subjects
Aspergillus niger - genetics
Aspergillus niger - metabolism
Biological Transport, Active - physiology
EPS
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Deletion
gene regulation
Genome-Wide Association Study
GEOSCIENCES
Hexuronic Acids - metabolism
Laboratorium voor Fytopathologie
Laboratorium voor Phytopathologie
Laboratory of Phytopathology
pectinase
Pectins - metabolism
polygalacturonic acid
Research Letter
Research Letters
Trans-Activators - genetics
Trans-Activators - metabolism
transcriptomics
Zn2Cys6 transcription factor
transcriptomics
Zn2Cys6 transcription factor
gene regulation
pectinase
polygalacturonic acid
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Abstract We identified the d‐galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome‐wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.
AbstractList We identified the d‐ galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger , which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome‐wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.
We identified the d‐galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome‐wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.
We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.
Author Aguilar Pontes, Maria Victoria
Visser, Jaap
Kowalczyk, Joanna E.
Alazi, Ebru
Ram, Arthur F. J.
Peng, Mao
Kan, Jan A. L.
Vries, Ronald P.
Niu, Jing
AuthorAffiliation 3 Laboratory of Phytopathology Wageningen University The Netherlands
1 Molecular Microbiology and Biotechnology Institute of Biology Leiden Leiden University The Netherlands
2 Fungal Physiology CBS‐KNAW Fungal Biodiversity Centre & Fungal Molecular Physiology Utrecht University The Netherlands
AuthorAffiliation_xml – name: 3 Laboratory of Phytopathology Wageningen University The Netherlands
– name: 1 Molecular Microbiology and Biotechnology Institute of Biology Leiden Leiden University The Netherlands
– name: 2 Fungal Physiology CBS‐KNAW Fungal Biodiversity Centre & Fungal Molecular Physiology Utrecht University The Netherlands
Author_xml – sequence: 1
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  surname: Alazi
  fullname: Alazi, Ebru
  organization: Leiden University
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  surname: Ram
  fullname: Ram, Arthur F. J.
  organization: Leiden University
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Issue 12
Keywords transcriptomics
Zn2Cys6 transcription factor
gene regulation
pectinase
polygalacturonic acid
Language English
License Attribution-NonCommercial-NoDerivs
2016 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
These authors contributed equally to this work
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SSID ssj0001819
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Snippet We identified the d‐galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be...
We identified the d‐ galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger , which was found to be...
We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be...
We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be...
SourceID wageningen
pubmedcentral
osti
pubmed
crossref
wiley
SourceType Open Access Repository
Index Database
Enrichment Source
Publisher
StartPage 1804
SubjectTerms Aspergillus niger - genetics
Aspergillus niger - metabolism
Biological Transport, Active - physiology
EPS
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Deletion
gene regulation
Genome-Wide Association Study
GEOSCIENCES
Hexuronic Acids - metabolism
Laboratorium voor Fytopathologie
Laboratorium voor Phytopathologie
Laboratory of Phytopathology
pectinase
Pectins - metabolism
polygalacturonic acid
Research Letter
Research Letters
Trans-Activators - genetics
Trans-Activators - metabolism
transcriptomics
Zn2Cys6 transcription factor
Title The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2F1873-3468.12211
https://www.ncbi.nlm.nih.gov/pubmed/27174630
https://www.osti.gov/servlets/purl/1437993
https://pubmed.ncbi.nlm.nih.gov/PMC5111758
http://www.narcis.nl/publication/RecordID/oai:library.wur.nl:wurpubs%2F506380
Volume 590
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