ESAT-6/CFP-10 Fusion Protein and Peptides for Optimal Diagnosis of Mycobacterium tuberculosis Infection by Ex Vivo Enzyme-Linked Immunospot Assay in The Gambia

• Published in: Journal of Clinical Microbiology Vol. 43; no. 5; pp. 2070 - 2074
• Main Authors: Hill, Philip C, Jackson-Sillah, Dolly, Fox, Annette, Franken, Kees L. M. C, Lugos, Moses D, Jeffries, David J, Donkor, Simon A, Hammond, Abdulrahman S, Adegbola, Richard A, Ottenhoff, Tom H. M, Klein, Michel R, Brookes, Roger H
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.05.2005
• Subjects: Antigens, Bacterial - analysis; Antigens, Bacterial - genetics; Bacterial Proteins - analysis; Bacterial Proteins - genetics; Biological and medical sciences; Enzyme-Linked Immunosorbent Assay; Ethnicity; Female; Fundamental and applied biological sciences. Psychology; Gambia; Housing; Humans; Infectious diseases; Male; Medical sciences; Microbiology; Mycobacteriology and Aerobic Actinomycetes; Mycobacterium tuberculosis; Mycobacterium tuberculosis - isolation & purification; Peptide Fragments - analysis; Recombinant Fusion Proteins - analysis; Tuberculosis - diagnosis; Tuberculosis - transmission; Gambia; Africa; Microbiology; Peptides; Enzyme; Mycobacterial infection; Infection; Mycobacterium tuberculosis; Mycobacteriales; Bacteriosis; Mycobacteriaceae; Bacteria; Actinomycetes; Diagnosis; Fusion protein
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/jcm.43.5.2070-2074.2005

Overlapping peptides of Mycobacterium tuberculosis antigens ESAT-6 and CFP-10 offer increased specificity over the purified protein derivative skin test when they were used in an ex vivo enzyme-linked immunospot (ELISPOT) assay for gamma interferon detection for the diagnosis of M. tuberculosis infe...