Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration
Collective cell migration is central to many developmental and pathological processes. However, the mechanisms that keep cell collectives together and coordinate movement of multiple cells are poorly understood. Using the border cell migration model, we find that Protein phosphatase 1 (Pp1) activity...
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Published in | eLife Vol. 9 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
eLife Science Publications, Ltd
05.05.2020
eLife Sciences Publications Ltd eLife Sciences Publication eLife Sciences Publications, Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Collective cell migration is central to many developmental and pathological processes. However, the mechanisms that keep cell collectives together and coordinate movement of multiple cells are poorly understood. Using the
border cell migration model, we find that Protein phosphatase 1 (Pp1) activity controls collective cell cohesion and migration. Inhibition of Pp1 causes border cells to round up, dissociate, and move as single cells with altered motility. We present evidence that Pp1 promotes proper levels of cadherin-catenin complex proteins at cell-cell junctions within the cluster to keep border cells together. Pp1 further restricts actomyosin contractility to the cluster periphery rather than at individual internal border cell contacts. We show that the myosin phosphatase Pp1 complex, which inhibits non-muscle myosin-II (Myo-II) activity, coordinates border cell shape and cluster cohesion. Given the high conservation of Pp1 complexes, this study identifies Pp1 as a major regulator of collective versus single cell migration. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23 Institute of Metabolic and Cardiovascular Diseases (I2MC), Université de Toulouse, Institut National de la Santé et de la Recherche Médicale (INSERM) UMR1048, Toulouse, France. Division of Immunity and Pathogenesis, Burnett School of Biomedical, Sciences, University of Central Florida College of Medicine, Orlando, United States. |
ISSN: | 2050-084X 2050-084X |
DOI: | 10.7554/eLife.52979 |