shRNA-mediated AMBRA1 knockdown reduces the cisplatin-induced autophagy and sensitizes ovarian cancer cells to cisplatin

• Published in: Journal of toxicological sciences Vol. 41; no. 1; pp. 45 - 53
• Main Authors: Hou, Xiaoman, Tian, Yongjie, Lin, Xueyan, Zhang, Lijuan, Yu, Lili, Wei, Wei, Li, Xiaoyan
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Society of Toxicology 01.02.2016; Japan Science and Technology Agency
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - physiology; Ambra1; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Apoptosis - drug effects; Apoptosis - genetics; Autophagy; Autophagy - drug effects; Autophagy - genetics; Cell Line, Tumor; Cisplatin; Cisplatin - pharmacology; Cisplatin - therapeutic use; Cisplatin sensitivity; Drug Resistance, Neoplasm - drug effects; Drug Resistance, Neoplasm - genetics; Female; Gene Knockdown Techniques; Humans; Microtubule-Associated Proteins - metabolism; Molecular Targeted Therapy; Ovarian cancer; Ovarian Neoplasms - drug therapy; Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - pathology; Phagosomes - metabolism; RNA, Small Interfering - genetics; RNA, Small Interfering - physiology; Transfection

Recent research has revealed a role for Ambra1, an autophagy-related gene-related (ATG) protein, in the autophagic pro-survival response, and Ambra1 has been shown to regulate Beclin1 and Beclin1-dependent autophagy in embryonic stem cells and cancer cells. However, whether Ambra1 plays an important...