Step‐by‐step protocol for the isolation and transient transformation of hornwort protoplasts

• Published in: Applications in plant sciences Vol. 10; no. 2; pp. e11456 - n/a
• Main Authors: Neubauer, Anna, Ruaud, Stéphanie, Waller, Manuel, Frangedakis, Eftychios, Li, Fay‐Wei, Nötzold, Svenja I., Wicke, Susann, Bailly, Aurélien, Szövényi, Péter
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.03.2022; John Wiley and Sons Inc; Wiley
• Subjects: Anthoceros; Anthoceros agrestis; Cell division; Cell walls; Chloroplasts; Comparative analysis; Flowers & plants; Genetic transformation; genome; Genomes; hornworts; Life cycles; liquids; Localization; model organism; Plant sciences; Polyethylene glycol; Proteins; Protocol; Protocol Note; Protoplasts; transient transformation; hornworts; transient transformation; model organism; Anthoceros; protoplasts

Premise A detailed protocol for the protoplast transformation of hornwort tissue is not yet available, limiting molecular biological investigations of these plants and comparative analyses with other bryophytes, which display a gametophyte‐dominant life cycle and are critical to understanding the ev...