人源性TAT—Fab抗体的表达优化及生物活性测定
为了提高抗体的表达水平，获得有保护作用的人源性破伤风抗毒素Fab（TAT-Fab）抗体，对人源性TAT-Fab抗体在大肠杆菌中的表达条件进行优化并测定其体内外生物学活性．ELISA显示所表达的TAT—Fab抗体可特异性结合破伤风外毒素，且优化后的表达水平较优化前提高近5倍；表达产物经超滤分离浓缩后，Western—blot显示在分子量约50 kDa处有一TAT-Fab抗体的特异性表达条带；小鼠保护实验显示其可特异性中和破伤风外毒素，明显延长小鼠的生存时间．以上结果表明所制备的人源性TAT-Fab抗体在体内外均具有中和破伤风外毒素的效应，为人源性破伤风抗毒素的研制和生产提供了实验依据．...
Saved in:
| Published in | Zhejiang daxue xue bao. Journal of Zhejiang University. Agriculture and life sciences. Nong ye yu sheng ming ke xue ban Vol. 33; no. 6; pp. 651 - 655 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
山东大学,医学院,免疫学研究所,山东,济南,250012%山东大学第二医院,临床分子生物学实验室,山东,济南,250033
2007
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | 为了提高抗体的表达水平，获得有保护作用的人源性破伤风抗毒素Fab（TAT-Fab）抗体，对人源性TAT-Fab抗体在大肠杆菌中的表达条件进行优化并测定其体内外生物学活性．ELISA显示所表达的TAT—Fab抗体可特异性结合破伤风外毒素，且优化后的表达水平较优化前提高近5倍；表达产物经超滤分离浓缩后，Western—blot显示在分子量约50 kDa处有一TAT-Fab抗体的特异性表达条带；小鼠保护实验显示其可特异性中和破伤风外毒素，明显延长小鼠的生存时间．以上结果表明所制备的人源性TAT-Fab抗体在体内外均具有中和破伤风外毒素的效应，为人源性破伤风抗毒素的研制和生产提供了实验依据． |
|---|---|
| AbstractList | R392.11; 为了提高抗体的表达水平,获得有保护作用的人源性破伤风抗毒素Fab(TAT-Fab)抗体,对人源性TAT-Fab抗体在大肠杆菌中的表达条件进行优化并测定其体内外生物学活性.ELISA显示所表达的TAT-Fab抗体可特异性结合破伤风外毒素,且优化后的表达水平较优化前提高近5倍;表达产物经超滤分离浓缩后,Western-blot显示在分子量约50 kDa处有一TAT-Fab抗体的特异性表达条带;小鼠保护实验显示其可特异性中和破伤风外毒素,明显延长小鼠的生存时间.以上结果表明所制备的人源性TAT-Fab抗体在体内外均具有中和破伤风外毒素的效应,为人源性破伤风抗毒素的研制和生产提供了实验依据. 为了提高抗体的表达水平，获得有保护作用的人源性破伤风抗毒素Fab（TAT-Fab）抗体，对人源性TAT-Fab抗体在大肠杆菌中的表达条件进行优化并测定其体内外生物学活性．ELISA显示所表达的TAT—Fab抗体可特异性结合破伤风外毒素，且优化后的表达水平较优化前提高近5倍；表达产物经超滤分离浓缩后，Western—blot显示在分子量约50 kDa处有一TAT-Fab抗体的特异性表达条带；小鼠保护实验显示其可特异性中和破伤风外毒素，明显延长小鼠的生存时间．以上结果表明所制备的人源性TAT-Fab抗体在体内外均具有中和破伤风外毒素的效应，为人源性破伤风抗毒素的研制和生产提供了实验依据． |
| Author | 王群 刘军莉 高飞 张蘋 张利宁 |
| AuthorAffiliation | 山东大学医学院免疫学研究所,山东济南250012 山东大学第二医院临床分子生物学实验室,山东济南250033 |
| AuthorAffiliation_xml | – name: 山东大学,医学院,免疫学研究所,山东,济南,250012%山东大学第二医院,临床分子生物学实验室,山东,济南,250033 |
| Author_FL | WANG Qun GAO Fei LIU Jun-li ZHANG Pin ZHANG Li-ning |
| Author_FL_xml | – sequence: 1 fullname: WANG Qun – sequence: 2 fullname: LIU Jun-li – sequence: 3 fullname: GAO Fei – sequence: 4 fullname: ZHANG Pin – sequence: 5 fullname: ZHANG Li-ning |
| Author_xml | – sequence: 1 fullname: 王群 刘军莉 高飞 张蘋 张利宁 |
| BookMark | eNo9j79Lw0AcxW-oYK39J8Q18X4kl5y4lGJVKLhkD3eXpCbWKxrE1qmDi62gYFHpIk7q4qRL1f4x0qT9M7xScXp8H5_ve7wVUFAtFQKwjqBJCEYbiRmnqdpEELoGw5CZGELHhNSECBdA8d9fBuU0jQWEGFNCKSqCrclolI9u8u6zV_F-uoMaF3nvfvJ9Ox1ezJ5eZuPx5Oshu7rLrnvTweP08jV__9Rw_tHP3oarYCnizTQs_2kJeLVtr7pr1Pd39qqVuiGpjQ0kXGLx0GEOsiWGWl3LErZLHRIgGRB9CGGzAEspIhpxRF0RUCwkY5RJapESMBaxZ1xFXDX8pHV6onShf54E7bbwVaeTHs0nQ6oHa35twcuDlmocx_pDcHkYxc3QxzabF1PyCyxDbrI |
| ClassificationCodes | R392.11 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W91 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3321/j.issn:1008-9209.2007.06.012 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库-医药卫生 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Optimization of human TAT-Fab antibody expression and its bioactivity assay |
| DocumentTitle_FL | Optimization of human TAT-Fab antibody expression and its bioactivity assay |
| EndPage | 655 |
| ExternalDocumentID | zjdxxb_nyysm200706012 25986736 |
| GrantInformation_xml | – fundername: 国家高技术研究发展计划(863计划) funderid: (2002AA2Z3338) |
| GroupedDBID | 2RA 5VS 92L ALMA_UNASSIGNED_HOLDINGS CQIGP CUKSK GROUPED_DOAJ W91 ~WA 2B. 4A8 92I 93N PSX TCJ |
| ID | FETCH-LOGICAL-c652-1b834ae79715c20797844b58673d1cd344bbb59d2ccbf6fa168bd62bc9969c643 |
| ISSN | 1008-9209 |
| IngestDate | Thu May 29 03:58:31 EDT 2025 Thu Nov 24 20:29:27 EST 2022 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 6 |
| Keywords | 抗体 Fab 噬菌体展示 破伤风抗毒素 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c652-1b834ae79715c20797844b58673d1cd344bbb59d2ccbf6fa168bd62bc9969c643 |
| Notes | antibody tetanus anti-toxin; phage display; Fab; antibody R392.11 phage display Fab tetanus anti-toxin 33-1247/S |
| PageCount | 5 |
| ParticipantIDs | wanfang_journals_zjdxxb_nyysm200706012 chongqing_backfile_25986736 |
| PublicationCentury | 2000 |
| PublicationDate | 2007 |
| PublicationDateYYYYMMDD | 2007-01-01 |
| PublicationDate_xml | – year: 2007 text: 2007 |
| PublicationDecade | 2000 |
| PublicationTitle | Zhejiang daxue xue bao. Journal of Zhejiang University. Agriculture and life sciences. Nong ye yu sheng ming ke xue ban |
| PublicationTitleAlternate | Journal of Zhejiang University(Agriculture & Life Sciences) |
| PublicationTitle_FL | JOURNAL OF ZHEJIANG UNIVERSITY(AGRICULTURE AND LIFE SCIENCES) |
| PublicationYear | 2007 |
| Publisher | 山东大学,医学院,免疫学研究所,山东,济南,250012%山东大学第二医院,临床分子生物学实验室,山东,济南,250033 |
| Publisher_xml | – name: 山东大学,医学院,免疫学研究所,山东,济南,250012%山东大学第二医院,临床分子生物学实验室,山东,济南,250033 |
| SSID | ssib002263661 ssib012436859 ssib001051300 ssib046628674 ssib001101329 ssib006567744 ssj0001549748 ssib001066910 ssib002040690 ssib002807042 ssib000968931 ssib006704888 ssib008679797 ssib051373729 |
| Score | 1.6898601 |
| Snippet | ... R392.11;... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 651 |
| SubjectTerms | Fab 噬菌体展示 抗体 破伤风抗毒素 |
| Title | 人源性TAT—Fab抗体的表达优化及生物活性测定 |
| URI | http://lib.cqvip.com/qk/94288A/20076/25986736.html https://d.wanfangdata.com.cn/periodical/zjdxxb-nyysm200706012 |
| Volume | 33 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR1Na9RANLQVxIsoKtZa6aHjJeyaTSaTGfGSrVmKQkFYoXhZMvno2totdrvQ7akHL1ZBwaLSi3hSL570UrU_Rtptf4bvTWZ3R1hEhWz2ZfIyk3kfM28y895Y1iw0irGXClGqxKBulPugc3kclzKeCOkKxoNMrfJdYPP36Z1Ff3FsfNpYtdTZkOVka6Rfyf9wFdKAr-gl-w-cHWQKCQADf-EMHIbzX_GYRJRUQzwihmfhIMAdEgb1sN5fx0BrsVTpgBCoR24T4ZEoICIknJKIk7BCQo5ANcIDceaIgBSfcACYAmqYAz5FiaghwAUJhSoa8KuDolUK4FfxqTCCUkwL-EEzWwaRXLLTeLOT2fiT8VrZNizjAcpw1UjZDpfWdZiQYsLj0cM8s3X_3S7bC7hnUjezux273cwAXsVvICv9AgYqoN47Ui8XYFVDaqvK8X51GRFVpAQiCbgHdayqewB4REQKHagTOogFN3jVTFJZIVVU1Yt9YgZfVQKjC8AFIcJ1hNlHFME6tC6YDT7T4XIzfeWP6pY8zy26JSxAxcjQRegQmqzs6LXkv4f-djF4fuCxceuUC6Mg7Hfu3jOsZ1AlYUbXgwbWc8xJT8ZMaxAsvYpnWsMOuj2bbtDMY6abM4f-gA6tXzD9A3OzBBZg8z98H4zdGAwnpcFyxN0NBuVRhm7QerRROPFTGM0WzqqaIKetWU2vGwW1bo6kFQZCaYJcPQZRUt52rRyk0jAM6-ess1puZ8JCPc9bY1vNC9atw_393v7L3vYHUMSf27uggr2dN4c_Xh3vPTl5__Hk4ODw-9uj56-PXuwc7747fvqp9-UbIPe-Pjv6vHfRqtei-tx8SW9UUkoYNG4VyT0aZ1D1ip-4DvxzSqWPrEsrSerBhZS-SN0kkTnL4wrjMmWuTIRgIoEhwSVrorXWyi5bM15Kk9yjuaRJQuMYMNLUkSLzMibcXPiT1tSg4mDnJisYva3Rl5NJ67omRUO3Uu3G1nK6uSkbrW63vYoUxPhL7pU_ZjNlnSlmMPBD41VrYmO9k02D6b0hrykB_AXTJ7Ef |
| linkProvider | Colorado Alliance of Research Libraries |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E4%BA%BA%E6%BA%90%E6%80%A7TAT%E2%80%94Fab%E6%8A%97%E4%BD%93%E7%9A%84%E8%A1%A8%E8%BE%BE%E4%BC%98%E5%8C%96%E5%8F%8A%E7%94%9F%E7%89%A9%E6%B4%BB%E6%80%A7%E6%B5%8B%E5%AE%9A&rft.jtitle=Zhejiang+daxue+xue+bao.+Journal+of+Zhejiang+University.+Agriculture+and+life+sciences.+Nong+ye+yu+sheng+ming+ke+xue+ban&rft.au=%E7%8E%8B%E7%BE%A4+%E5%88%98%E5%86%9B%E8%8E%89+%E9%AB%98%E9%A3%9E+%E5%BC%A0%E8%98%8B+%E5%BC%A0%E5%88%A9%E5%AE%81&rft.date=2007&rft.issn=1008-9209&rft.volume=33&rft.issue=6&rft.spage=651&rft.epage=655&rft_id=info:doi/10.3321%2Fj.issn%3A1008-9209.2007.06.012&rft.externalDocID=25986736 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F94288A%2F94288A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzjdxxb-nyysm%2Fzjdxxb-nyysm.jpg |