Quality control despite mistranslation caused by an ambiguous genetic code

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 105; no. 43; pp. 16502 - 16507
• Main Authors: Ruan, Benfang, Palioura, Sotiria, Sabina, Jeffrey, Marvin-Guy, Laure, Kochhar, Sunil, LaRossa, Robert A, Söll, Dieter
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 28.10.2008; National Acad Sciences
• Subjects: active sites; Alleles; Amino acids; aminoacyl tRNA ligases; Aminoacyl-tRNA ligase; Biological Sciences; Codons; E coli; Enzymes; Escherichia coli; Escherichia coli - genetics; Genetic Code; Genetic mutation; Genetics; Heat shock; heat shock response; Heat-Shock Response - physiology; Mass Spectrometry; Mass spectroscopy; Messenger RNA; Missense mutation; mutants; Mutation, Missense; polypeptides; Protein Biosynthesis; Protein synthesis; Proteins; Quality control; RNA, Transfer, Amino Acyl - physiology; Survival; Transfer RNA; Translation; translation (genetics); tRNA

A high level of accuracy during protein synthesis is considered essential for life. Aminoacyl-tRNA synthetases (aaRSs) translate the genetic code by ensuring the correct pairing of amino acids with their cognate tRNAs. Because some aaRSs also produce misacylated aminoacyl-tRNA (aa-tRNA) in vivo, we...