Suitability of internal transcribed spacers (ITS) as markers for the population genetic structure of Blastocystis spp

• Published in: Parasites & vectors Vol. 7; no. 1; p. 461
• Main Authors: Villalobos, Guiehdani, Orozco-Mosqueda, Guadalupe Erendira, Lopez-Perez, Merle, Lopez-Escamilla, Eduardo, Córdoba-Aguilar, Alex, Rangel-Gamboa, Lucia, Olivo-Diaz, Angelica, Romero-Valdovinos, Mirza, Maravilla, Pablo, Martinez-Hernandez, Fernando
• Format: Journal Article
• Language: English
• Published: England Springer-Verlag 03.10.2014; BioMed Central Ltd; BioMed Central; BMC
• Subjects: Adolescent; Base Sequence; Biological diversity; Blastocystis; Blastocystis - genetics; Blastocystis - isolation & purification; Blastocystis Infections - parasitology; Blastocystis spp; Blastocystis subtypes; Child; Child, Preschool; children; Cluster Analysis; Deoxyribonucleic acid; DNA; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; Evolution; feces; Feces - parasitology; Female; gastrointestinal system; Gene Flow; Genes; Genetic aspects; Genetic diversity; Genetic markers; Genetic Markers - genetics; Genetic Variation; Genetics, Population; Haplotypes; Humans; Infant; Internal transcribed spacers; Male; Mexico; Molecular Sequence Data; Parasites; phylogeny; polymerase chain reaction; ribosomal DNA; Sequence Analysis, DNA; Short Report; Software; Values; Libya; Mexico

BACKGROUND: The purpose of this study was to assess the genetic variation and differentiation of Blastocystis subtypes (STs) recovered from symptomatic children by analysing partial sequences of the small subunit rDNA gene region (SSUrDNA) and internal transcribed spacers (1 and 2) plus the 5.8S reg...