Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles
West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required....
Saved in:
| Published in | Scientific reports Vol. 11; no. 1; pp. 9213 - 10 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
London
Nature Publishing Group UK
28.04.2021
Nature Publishing Group Nature Portfolio |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required. The coexpression of prM protein and E protein leads to the secretion of subviral particles (SPs). Deletion of the C-terminal region of E protein is reported to affect the production of SPs by some flaviviruses. However, the influence of such a deletion on the properties and antigenicity of WNV E protein is unclear. We analyzed the properties of full-length E protein and E proteins lacking the C-terminal region as novel serodiagnostics for WNV infection. Deletion of the C-terminal region of E protein suppressed the formation of SPs but did not affect the production of E protein. The sensitivity of an enzyme-linked immunosorbent assay (ELISA) using the full-length E protein was higher than that using the truncated E proteins. Furthermore, in the ELISA using full-length E protein, there was little cross-reactivity with anti-JEV antibodies, and the sensitivity was similar to that of the neutralization test. |
|---|---|
| AbstractList | West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required. The coexpression of prM protein and E protein leads to the secretion of subviral particles (SPs). Deletion of the C-terminal region of E protein is reported to affect the production of SPs by some flaviviruses. However, the influence of such a deletion on the properties and antigenicity of WNV E protein is unclear. We analyzed the properties of full-length E protein and E proteins lacking the C-terminal region as novel serodiagnostics for WNV infection. Deletion of the C-terminal region of E protein suppressed the formation of SPs but did not affect the production of E protein. The sensitivity of an enzyme-linked immunosorbent assay (ELISA) using the full-length E protein was higher than that using the truncated E proteins. Furthermore, in the ELISA using full-length E protein, there was little cross-reactivity with anti-JEV antibodies, and the sensitivity was similar to that of the neutralization test. Abstract West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required. The coexpression of prM protein and E protein leads to the secretion of subviral particles (SPs). Deletion of the C-terminal region of E protein is reported to affect the production of SPs by some flaviviruses. However, the influence of such a deletion on the properties and antigenicity of WNV E protein is unclear. We analyzed the properties of full-length E protein and E proteins lacking the C-terminal region as novel serodiagnostics for WNV infection. Deletion of the C-terminal region of E protein suppressed the formation of SPs but did not affect the production of E protein. The sensitivity of an enzyme-linked immunosorbent assay (ELISA) using the full-length E protein was higher than that using the truncated E proteins. Furthermore, in the ELISA using full-length E protein, there was little cross-reactivity with anti-JEV antibodies, and the sensitivity was similar to that of the neutralization test. West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required. The coexpression of prM protein and E protein leads to the secretion of subviral particles (SPs). Deletion of the C-terminal region of E protein is reported to affect the production of SPs by some flaviviruses. However, the influence of such a deletion on the properties and antigenicity of WNV E protein is unclear. We analyzed the properties of full-length E protein and E proteins lacking the C-terminal region as novel serodiagnostics for WNV infection. Deletion of the C-terminal region of E protein suppressed the formation of SPs but did not affect the production of E protein. The sensitivity of an enzyme-linked immunosorbent assay (ELISA) using the full-length E protein was higher than that using the truncated E proteins. Furthermore, in the ELISA using full-length E protein, there was little cross-reactivity with anti-JEV antibodies, and the sensitivity was similar to that of the neutralization test.West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required. The coexpression of prM protein and E protein leads to the secretion of subviral particles (SPs). Deletion of the C-terminal region of E protein is reported to affect the production of SPs by some flaviviruses. However, the influence of such a deletion on the properties and antigenicity of WNV E protein is unclear. We analyzed the properties of full-length E protein and E proteins lacking the C-terminal region as novel serodiagnostics for WNV infection. Deletion of the C-terminal region of E protein suppressed the formation of SPs but did not affect the production of E protein. The sensitivity of an enzyme-linked immunosorbent assay (ELISA) using the full-length E protein was higher than that using the truncated E proteins. Furthermore, in the ELISA using full-length E protein, there was little cross-reactivity with anti-JEV antibodies, and the sensitivity was similar to that of the neutralization test. |
| ArticleNumber | 9213 |
| Author | Tabata, Koshiro Yoshii, Kentaro Kobayashi, Shintaro Maezono, Keisuke Kariwa, Hiroaki |
| Author_xml | – sequence: 1 givenname: Keisuke surname: Maezono fullname: Maezono, Keisuke organization: Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University – sequence: 2 givenname: Shintaro surname: Kobayashi fullname: Kobayashi, Shintaro email: shin-kobayashi@vetmed.hokudai.ac.jp organization: Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University – sequence: 3 givenname: Koshiro surname: Tabata fullname: Tabata, Koshiro organization: 2Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University – sequence: 4 givenname: Kentaro surname: Yoshii fullname: Yoshii, Kentaro organization: Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University, National Research Center for the Control and Prevention of Infectious Diseases, Nagasaki University – sequence: 5 givenname: Hiroaki surname: Kariwa fullname: Kariwa, Hiroaki organization: Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33911132$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9Uk1v1DAQjVAR_aB_gAOyxIVLwF9J7AtSVUpZaQUHQBwt25lkvcrai52s1H-Pd7OUtof6YI_t957fjOe8OPHBQ1G8IfgDwUx8TJxUUpSYklKIpmnK6kVxRjGvSsooPXkQnxaXKa1xHhWVnMhXxSljkhDC6FkxfIYdDGG7AT-i0CGNVq5fDXcobcG6zlmUIIbW6d6HNObtzXLx4wp1IaLfkEb0zQ2Adi5OCTnfgR1d8GhKzvcoTSZf6AFtdczMAdLr4mWnhwSXx_Wi-PXl5uf113L5_XZxfbUsbV3hsZS8ZVyTruacNdx0wojWYKytAG0aqDBpODDT8AryzoLoTGuYrLDBsoEa2EWxmHXboNdqG91GxzsVtFOHgxB7dbSkMMHUCto0WhgOAoSQhtDa8hwAxzJrfZq1tpPZQGtznXJOj0Qf33i3Un3YKYEFrmWVBd4fBWL4M-WaqY1LFoZBewhTUrQiUpCcUp2h755A12GKPpfqgCKc5Tmj3j50dG_l36dmAJ0BNoaUInT3EILVvnnU3DwqN486NI_a2xRPSNaNev-dOSs3PE9lMzXld3wP8b_tZ1h_AdqA2TE |
| CitedBy_id | crossref_primary_10_1007_s00253_023_12817_5 crossref_primary_10_1016_j_isci_2024_109539 crossref_primary_10_3390_tropicalmed8040224 |
| Cites_doi | 10.1038/s41598-020-64199-7 10.1128/JVI.79.21.13262-13274.2005 10.1038/nsb990 10.1016/j.biologicals.2010.07.004 10.1099/0022-1317-70-1-37 10.1128/CMR.00045-12 10.1038/ncomms14722 10.1016/0042-6822(92)90317-i 10.1371/journal.pntd.0005936 10.1155/2015/376230 10.1128/JVI.02458-10 10.1128/JVI.70.7.4549-4557.1996 10.1016/0264-410x(94)90206-2 10.1006/meth.2000.1031 10.1128/JVI.73.7.5605-5612.1999 10.1590/0074-02760180332 10.1128/JCM.38.5.1823-1826.2000 10.1292/jvms.09-0311 10.1074/jbc.M115.712760 10.1099/vir.0.80247-0 10.1371/journal.ppat.1008238 10.1371/journal.pone.0100641 10.1016/s0065-3527(00)55005-2 10.1128/JVI.00080-06 10.1016/j.virusres.2017.10.015 10.1016/s0065-3527(03)61001-8 10.1128/JCM.42.9.4101-4110.2004 10.1038/375291a0 10.1128/jvi.77.2.813-820.2003 10.3390/v6010013 10.1016/s0065-3527(03)61005-5 10.1016/0042-6822(91)90034-9 10.1128/JVI.01732-06 10.3201/eid0701.010118 10.1016/j.ttbdis.2016.03.002 10.1073/pnas.1011036107 10.3121/cmr.1.1.5 10.1016/S1286-4579(02)01647-7 10.1038/s41598-017-10595-5 10.1128/JVI.01283-13 10.1128/JCM.02488-13 10.1038/s41467-020-16925-y 10.1128/JVI.78.22.12591-12602.2004 10.1111/j.1440-1789.2011.01275.x 10.1128/jvi.77.21.11357-11366.2003 10.1128/JVI.69.9.5816-5820.1995 10.1038/ncomms7341 10.1016/0264-410X(95)00133-L 10.4269/ajtmh.2011.09-0714 |
| ContentType | Journal Article |
| Copyright | The Author(s) 2021 The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: The Author(s) 2021 – notice: The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | C6C AAYXX CITATION NPM 3V. 7X7 7XB 88A 88E 88I 8FE 8FH 8FI 8FJ 8FK ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M2P M7P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS Q9U 7X8 5PM DOA |
| DOI | 10.1038/s41598-021-88777-5 |
| DatabaseName | Springer Nature OA Free Journals CrossRef PubMed ProQuest Central (Corporate) ProQuest Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest One Sustainability (subscription) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One ProQuest Central Korea Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni) Medical Database Science Database Biological Science Database ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central China ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Sustainability ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | Publicly Available Content Database PubMed CrossRef MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: C6C name: Springer Nature OA Free Journals url: http://www.springeropen.com/ sourceTypes: Publisher – sequence: 2 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 3 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 4 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2045-2322 |
| EndPage | 10 |
| ExternalDocumentID | oai_doaj_org_article_0102c8277a8b4e8e889b126c4889e409 PMC8080695 33911132 10_1038_s41598_021_88777_5 |
| Genre | Journal Article |
| GrantInformation_xml | – fundername: Research Program on Emerging and Re-emerging Infectious Diseases from Japan Agency for Medical Research and Development, AMED grantid: 19fk0108036h0003 – fundername: Takeda Science Foundation funderid: http://dx.doi.org/10.13039/100007449 – fundername: Japan Society for the Promotion of Science grantid: 18K14574; 17H03910 funderid: http://dx.doi.org/10.13039/501100001691 – fundername: Kanehara Ichiro Memorial Foundation – fundername: Japan Society for the Promotion of Science grantid: 17H03910 – fundername: Japan Society for the Promotion of Science grantid: 18K14574 – fundername: ; – fundername: ; grantid: 18K14574; 17H03910 – fundername: ; grantid: 19fk0108036h0003 |
| GroupedDBID | 0R~ 3V. 4.4 53G 5VS 7X7 88A 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAJSJ AAKDD ABDBF ABUWG ACGFS ACSMW ACUHS ADBBV ADRAZ AENEX AEUYN AFKRA AJTQC ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI C6C CCPQU DIK DWQXO EBD EBLON EBS ESX FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE KQ8 LK8 M0L M1P M2P M48 M7P M~E NAO OK1 PIMPY PQQKQ PROAC PSQYO RNT RNTTT RPM SNYQT UKHRP AASML AAYXX AFPKN CITATION PHGZM PHGZT NPM PJZUB PPXIY PQGLB 7XB 8FK AARCD K9. PKEHL PQEST PQUKI PRINS Q9U 7X8 5PM PUEGO |
| ID | FETCH-LOGICAL-c650t-94d34a1f644374bf8b8db00ac8eab7e50174e3b745e7e5ce8fbdb3950b097e6e3 |
| IEDL.DBID | M48 |
| ISSN | 2045-2322 |
| IngestDate | Wed Aug 27 01:21:59 EDT 2025 Thu Aug 21 18:15:32 EDT 2025 Fri Jul 11 09:22:31 EDT 2025 Wed Aug 13 06:11:33 EDT 2025 Mon Jul 21 05:48:43 EDT 2025 Thu Apr 24 23:44:06 EDT 2025 Tue Jul 01 01:08:02 EDT 2025 Fri Feb 21 02:39:45 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Language | English |
| License | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c650t-94d34a1f644374bf8b8db00ac8eab7e50174e3b745e7e5ce8fbdb3950b097e6e3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | https://www.proquest.com/docview/2519143519?pq-origsite=%requestingapplication% |
| PMID | 33911132 |
| PQID | 2519143519 |
| PQPubID | 2041939 |
| PageCount | 10 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_0102c8277a8b4e8e889b126c4889e409 pubmedcentral_primary_oai_pubmedcentral_nih_gov_8080695 proquest_miscellaneous_2519811746 proquest_journals_2519143519 pubmed_primary_33911132 crossref_primary_10_1038_s41598_021_88777_5 crossref_citationtrail_10_1038_s41598_021_88777_5 springer_journals_10_1038_s41598_021_88777_5 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2021-04-28 |
| PublicationDateYYYYMMDD | 2021-04-28 |
| PublicationDate_xml | – month: 04 year: 2021 text: 2021-04-28 day: 28 |
| PublicationDecade | 2020 |
| PublicationPlace | London |
| PublicationPlace_xml | – name: London – name: England |
| PublicationTitle | Scientific reports |
| PublicationTitleAbbrev | Sci Rep |
| PublicationTitleAlternate | Sci Rep |
| PublicationYear | 2021 |
| Publisher | Nature Publishing Group UK Nature Publishing Group Nature Portfolio |
| Publisher_xml | – name: Nature Publishing Group UK – name: Nature Publishing Group – name: Nature Portfolio |
| References | Reed, Meece, Henkel, Shukla (CR8) 2003; 1 Sasaki (CR31) 2018; 243 Calisher (CR1) 1989; 70 Op De Beeck (CR43) 2003; 77 Komar (CR48) 2003; 61 Levanov (CR30) 2014; 52 Stiasny, Kiermayr, Holzmann, Heinz (CR37) 2006; 80 Heinz, Allison (CR15) 2000; 55 Colpitts, Conway, Montgomery, Fikrig (CR14) 2012; 25 Op De Beeck, Rouillé, Caron, Duvet, Dubuisson (CR23) 2004; 78 Chancey, Grinev, Volkova, Rios (CR3) 2015; 2015 Martin (CR12) 2000; 38 Han (CR22) 2017; 7 Butler (CR41) 2000; 22 Hanna (CR32) 2005; 79 Mason (CR46) 1991; 180 Morrone (CR36) 2020; 11 Kobayashi (CR51) 2020; 16 Chávez, Silva, Amarilla, Moraes Figueiredo (CR20) 2010; 38 Allison, Stiasny, Stadler, Mandl, Heinz (CR24) 1999; 73 Allison (CR44) 2003; 77 Kobayashi (CR50) 2016; 291 Ternovoĭ (CR7) 2006; 4 Stiasny, Kiermayr, Bernhart, Heinz (CR26) 2013; 87 Zhang (CR19) 2003; 10 Oliphant (CR18) 2006; 80 Hasan (CR35) 2017; 8 Beck (CR38) 2017; 11 Hsieh, Tsai, Nerurkar, Wang (CR42) 2014; 9 Holbrook, Shope, Barrett (CR21) 2004; 42 Pincus (CR47) 1992; 187 Rey, Heinz, Mandl, Kunz, Harrison (CR17) 1995; 375 Kobayashi, Orba, Yamaguchi, Kimura, Sawa (CR2) 2012; 32 Inagaki (CR11) 2016; 7 Allison, Stadler, Mandl, Kunz, Heinz (CR27) 1995; 69 Martins (CR4) 2019; 114 Kobayashi (CR28) 2020; 10 Platonov (CR5) 2001; 7 Fibriansah (CR33) 2015; 6 Fonseca, Pincus, Shope, Paoletti, Mason (CR45) 1994; 12 Kaufmann (CR34) 2010; 107 Brinton (CR16) 2013; 6 Murata (CR6) 2011; 84 Hirota, Nishi, Matsuda, Tsunemitsu, Shimiz (CR10) 2010; 72 Heinz (CR40) 1995; 13 Igarashi (CR9) 1992; 45 Schalich (CR29) 1996; 70 Kuno (CR13) 2003; 61 Shirato (CR49) 2004; 85 Fritz (CR25) 2011; 85 Koraka, Zeller, Niedrig, Osterhaus, Groen (CR39) 2002; 4 S Kobayashi (88777_CR50) 2016; 291 S Kobayashi (88777_CR2) 2012; 32 S Pincus (88777_CR47) 1992; 187 FX Heinz (88777_CR40) 1995; 13 FA Rey (88777_CR17) 1995; 375 K Shirato (88777_CR49) 2004; 85 SL Allison (88777_CR27) 1995; 69 DA Martin (88777_CR12) 2000; 38 SL Allison (88777_CR44) 2003; 77 C Chancey (88777_CR3) 2015; 2015 E Inagaki (88777_CR11) 2016; 7 SS Hasan (88777_CR35) 2017; 8 W Zhang (88777_CR19) 2003; 10 A Op De Beeck (88777_CR23) 2004; 78 LC Martins (88777_CR4) 2019; 114 R Murata (88777_CR6) 2011; 84 JE Butler (88777_CR41) 2000; 22 MA Brinton (88777_CR16) 2013; 6 C Beck (88777_CR38) 2017; 11 R Fritz (88777_CR25) 2011; 85 PW Mason (88777_CR46) 1991; 180 A Op De Beeck (88777_CR43) 2003; 77 VA Ternovoĭ (88777_CR7) 2006; 4 T Oliphant (88777_CR18) 2006; 80 TM Colpitts (88777_CR14) 2012; 25 FX Heinz (88777_CR15) 2000; 55 B Kaufmann (88777_CR34) 2010; 107 J Hirota (88777_CR10) 2010; 72 JF Han (88777_CR22) 2017; 7 P Koraka (88777_CR39) 2002; 4 JH Chávez (88777_CR20) 2010; 38 L Levanov (88777_CR30) 2014; 52 SR Morrone (88777_CR36) 2020; 11 S Kobayashi (88777_CR28) 2020; 10 A Igarashi (88777_CR9) 1992; 45 BA Fonseca (88777_CR45) 1994; 12 S Kobayashi (88777_CR51) 2020; 16 J Schalich (88777_CR29) 1996; 70 G Kuno (88777_CR13) 2003; 61 G Fibriansah (88777_CR33) 2015; 6 CH Calisher (88777_CR1) 1989; 70 AE Platonov (88777_CR5) 2001; 7 K Stiasny (88777_CR37) 2006; 80 SL Hanna (88777_CR32) 2005; 79 K Stiasny (88777_CR26) 2013; 87 SL Allison (88777_CR24) 1999; 73 SC Hsieh (88777_CR42) 2014; 9 KD Reed (88777_CR8) 2003; 1 N Komar (88777_CR48) 2003; 61 MR Holbrook (88777_CR21) 2004; 42 M Sasaki (88777_CR31) 2018; 243 |
| References_xml | – volume: 10 start-page: 7168 year: 2020 ident: CR28 article-title: Amino acid 159 of the envelope protein affects viral replication and T-cell infiltration by West Nile virus in intracranial infection publication-title: Sci. Rep. doi: 10.1038/s41598-020-64199-7 – volume: 79 start-page: 13262 year: 2005 end-page: 13274 ident: CR32 article-title: N-linked glycosylation of west nile virus envelope proteins influences particle assembly and infectivity publication-title: J. Virol. doi: 10.1128/JVI.79.21.13262-13274.2005 – volume: 10 start-page: 907 year: 2003 end-page: 912 ident: CR19 article-title: Visualization of membrane protein domains by cryo-electron microscopy of dengue virus publication-title: Nat. Struct. Biol. doi: 10.1038/nsb990 – volume: 38 start-page: 613 year: 2010 end-page: 618 ident: CR20 article-title: Domain III peptides from flavivirus envelope protein are useful antigens for serologic diagnosis and targets for immunization publication-title: Biologicals doi: 10.1016/j.biologicals.2010.07.004 – volume: 70 start-page: 37 issue: Pt 1 year: 1989 end-page: 43 ident: CR1 article-title: Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera publication-title: J. Gen. Virol. doi: 10.1099/0022-1317-70-1-37 – volume: 25 start-page: 635 year: 2012 end-page: 648 ident: CR14 article-title: West Nile Virus: Biology, transmission, and human infection publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.00045-12 – volume: 8 start-page: 14722 year: 2017 ident: CR35 article-title: A human antibody against Zika virus crosslinks the E protein to prevent infection publication-title: Nat. Commun. doi: 10.1038/ncomms14722 – volume: 187 start-page: 290 year: 1992 end-page: 297 ident: CR47 article-title: Recombinant vaccinia virus producing the prM and E proteins of yellow fever virus protects mice from lethal yellow fever encephalitis publication-title: Virology doi: 10.1016/0042-6822(92)90317-i – volume: 11 start-page: e0005936 year: 2017 ident: CR38 article-title: Improved reliability of serological tools for the diagnosis of West Nile fever in horses within Europe publication-title: PLoS Negl. Trop. Dis. doi: 10.1371/journal.pntd.0005936 – volume: 2015 start-page: 376230 year: 2015 ident: CR3 article-title: The global ecology and epidemiology of West Nile virus publication-title: Biomed. Res. Int. doi: 10.1155/2015/376230 – volume: 85 start-page: 4377 year: 2011 end-page: 4385 ident: CR25 article-title: The unique transmembrane hairpin of flavivirus fusion protein E is essential for membrane fusion publication-title: J. Virol. doi: 10.1128/JVI.02458-10 – volume: 45 start-page: 299 year: 1992 end-page: 305 ident: CR9 article-title: Epidemiology and control of Japanese encephalitis publication-title: World Health Stat. Q. – volume: 70 start-page: 4549 year: 1996 end-page: 4557 ident: CR29 article-title: Recombinant subviral particles from tick-borne encephalitis virus are fusogenic and provide a model system for studying flavivirus envelope glycoprotein functions publication-title: J. Virol. doi: 10.1128/JVI.70.7.4549-4557.1996 – volume: 12 start-page: 279 year: 1994 end-page: 285 ident: CR45 article-title: Recombinant vaccinia viruses co-expressing dengue-1 glycoproteins prM and E induce neutralizing antibodies in mice publication-title: Vaccine doi: 10.1016/0264-410x(94)90206-2 – volume: 22 start-page: 4 year: 2000 end-page: 23 ident: CR41 article-title: Solid supports in enzyme-linked immunosorbent assay and other solid-phase immunoassays publication-title: Methods doi: 10.1006/meth.2000.1031 – volume: 73 start-page: 5605 year: 1999 end-page: 5612 ident: CR24 article-title: Mapping of functional elements in the stem-anchor region of tick-borne encephalitis virus envelope protein E publication-title: J. Virol. doi: 10.1128/JVI.73.7.5605-5612.1999 – volume: 114 start-page: e180332 year: 2019 ident: CR4 article-title: First isolation of West Nile virus in Brazil publication-title: Mem. Inst. Oswaldo Cruz doi: 10.1590/0074-02760180332 – volume: 38 start-page: 1823 year: 2000 end-page: 1826 ident: CR12 article-title: Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.38.5.1823-1826.2000 – volume: 72 start-page: 369 year: 2010 end-page: 372 ident: CR10 article-title: Cross-reactivity of Japanese encephalitis virus-vaccinated horse sera in serodiagnosis of West Nile virus publication-title: J. Vet. Med. Sci. doi: 10.1292/jvms.09-0311 – volume: 291 start-page: 6559 year: 2016 end-page: 6568 ident: CR50 article-title: Rab8b regulates transport of West Nile virus particles from recycling endosomes publication-title: J. Biol. Chem. doi: 10.1074/jbc.M115.712760 – volume: 85 start-page: 3637 year: 2004 end-page: 3645 ident: CR49 article-title: Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus publication-title: J. Gen. Virol. doi: 10.1099/vir.0.80247-0 – volume: 16 start-page: e1008238 year: 2020 ident: CR51 article-title: West Nile virus capsid protein inhibits autophagy by AMP-activated protein kinase degradation in neurological disease development publication-title: PLoS Pathog. doi: 10.1371/journal.ppat.1008238 – volume: 9 start-page: e100641 year: 2014 ident: CR42 article-title: Characterization of the ectodomain of the envelope protein of dengue virus type 4: Expression, membrane association, secretion and particle formation in the absence of precursor membrane protein publication-title: PLoS ONE doi: 10.1371/journal.pone.0100641 – volume: 55 start-page: 231 year: 2000 end-page: 269 ident: CR15 article-title: Structures and mechanisms in flavivirus fusion publication-title: Adv. Virus Res. doi: 10.1016/s0065-3527(00)55005-2 – volume: 80 start-page: 9557 year: 2006 end-page: 9568 ident: CR37 article-title: Cryptic properties of a cluster of dominant flavivirus cross-reactive antigenic sites publication-title: J. Virol. doi: 10.1128/JVI.00080-06 – volume: 243 start-page: 69 year: 2018 end-page: 74 ident: CR31 article-title: Development of a rapid and quantitative method for the analysis of viral entry and release using a NanoLuc luciferase complementation assay publication-title: Virus Res. doi: 10.1016/j.virusres.2017.10.015 – volume: 61 start-page: 3 year: 2003 end-page: 65 ident: CR13 article-title: Serodiagnosis of flaviviral infections and vaccinations in humans publication-title: Adv. Virus Res. doi: 10.1016/s0065-3527(03)61001-8 – volume: 42 start-page: 4101 year: 2004 end-page: 4110 ident: CR21 article-title: Use of recombinant E protein domain III-based enzyme-linked immunosorbent assays for differentiation of tick-borne encephalitis serocomplex flaviviruses from mosquito-borne flaviviruses publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.42.9.4101-4110.2004 – volume: 375 start-page: 291 year: 1995 end-page: 298 ident: CR17 article-title: The envelope glycoprotein from tick-borne encephalitis virus at 2 A resolution publication-title: Nature doi: 10.1038/375291a0 – volume: 77 start-page: 813 year: 2003 end-page: 820 ident: CR43 article-title: Role of the transmembrane domains of prM and E proteins in the formation of yellow fever virus envelope publication-title: J. Virol. doi: 10.1128/jvi.77.2.813-820.2003 – volume: 6 start-page: 13 year: 2013 end-page: 53 ident: CR16 article-title: Replication cycle and molecular biology of the West Nile virus publication-title: Viruses doi: 10.3390/v6010013 – volume: 61 start-page: 185 year: 2003 end-page: 234 ident: CR48 article-title: West Nile virus: Epidemiology and ecology in North America publication-title: Adv. Virus Res. doi: 10.1016/s0065-3527(03)61005-5 – volume: 180 start-page: 294 year: 1991 end-page: 305 ident: CR46 article-title: Japanese encephalitis virus-vaccinia recombinants produce particulate forms of the structural membrane proteins and induce high levels of protection against lethal JEV infection publication-title: Virology doi: 10.1016/0042-6822(91)90034-9 – volume: 80 start-page: 12149 year: 2006 end-page: 12159 ident: CR18 article-title: Antibody recognition and neutralization determinants on domains I and II of West Nile Virus envelope protein publication-title: J. Virol. doi: 10.1128/JVI.01732-06 – volume: 7 start-page: 128 year: 2001 end-page: 132 ident: CR5 article-title: Outbreak of West Nile virus infection, Volgograd Region, Russia, 1999 publication-title: Emerg. Infect. Dis. doi: 10.3201/eid0701.010118 – volume: 7 start-page: 723 year: 2016 end-page: 729 ident: CR11 article-title: Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles publication-title: Ticks Tick Borne Dis. doi: 10.1016/j.ttbdis.2016.03.002 – volume: 107 start-page: 18950 year: 2010 end-page: 18955 ident: CR34 article-title: Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354 publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.1011036107 – volume: 1 start-page: 5 year: 2003 end-page: 12 ident: CR8 article-title: Birds, migration and emerging zoonoses: West Nile virus, lyme disease, influenza A and enteropathogens publication-title: Clin. Med. Res. doi: 10.3121/cmr.1.1.5 – volume: 4 start-page: 1209 year: 2002 end-page: 1215 ident: CR39 article-title: Reactivity of serum samples from patients with a flavivirus infection measured by immunofluorescence assay and ELISA publication-title: Microbes Infect. doi: 10.1016/S1286-4579(02)01647-7 – volume: 7 start-page: 10047 year: 2017 ident: CR22 article-title: Immunization with truncated envelope protein of Zika virus induces protective immune response in mice publication-title: Sci. Rep. doi: 10.1038/s41598-017-10595-5 – volume: 87 start-page: 9933 year: 2013 end-page: 9938 ident: CR26 article-title: The membrane-proximal “stem” region increases the stability of the flavivirus E protein postfusion trimer and modulates its structure publication-title: J. Virol. doi: 10.1128/JVI.01283-13 – volume: 52 start-page: 814 year: 2014 end-page: 822 ident: CR30 article-title: Diagnostic potential and antigenic properties of recombinant tick-borne encephalitis virus subviral particles expressed in mammalian cells from Semliki Forest virus replicons publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.02488-13 – volume: 11 start-page: 3112 year: 2020 ident: CR36 article-title: High flavivirus structural plasticity demonstrated by a non-spherical morphological variant publication-title: Nat. Commun. doi: 10.1038/s41467-020-16925-y – volume: 78 start-page: 12591 year: 2004 end-page: 12602 ident: CR23 article-title: The transmembrane domains of the prM and E proteins of yellow fever virus are endoplasmic reticulum localization signals publication-title: J. Virol. doi: 10.1128/JVI.78.22.12591-12602.2004 – volume: 32 start-page: 398 year: 2012 end-page: 405 ident: CR2 article-title: Accumulation of ubiquitinated proteins is related to West Nile virus-induced neuronal apoptosis publication-title: Neuropathology doi: 10.1111/j.1440-1789.2011.01275.x – volume: 77 start-page: 11357 year: 2003 end-page: 11366 ident: CR44 article-title: Two distinct size classes of immature and mature subviral particles from tick-borne encephalitis virus publication-title: J. Virol. doi: 10.1128/jvi.77.21.11357-11366.2003 – volume: 69 start-page: 5816 year: 1995 end-page: 5820 ident: CR27 article-title: Synthesis and secretion of recombinant tick-borne encephalitis virus protein E in soluble and particulate form publication-title: J. Virol. doi: 10.1128/JVI.69.9.5816-5820.1995 – volume: 6 start-page: 6341 year: 2015 ident: CR33 article-title: A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins publication-title: Nat. Commun. doi: 10.1038/ncomms7341 – volume: 13 start-page: 1636 year: 1995 end-page: 1642 ident: CR40 article-title: Recombinant and virion-derived soluble and particulate immunogens for vaccination against tick-borne encephalitis publication-title: Vaccine doi: 10.1016/0264-410X(95)00133-L – volume: 84 start-page: 461 year: 2011 end-page: 465 ident: CR6 article-title: Seroprevalence of West Nile virus in wild birds in far eastern Russia using a focus reduction neutralization test publication-title: Am. J. Trop. Med. Hyg. doi: 10.4269/ajtmh.2011.09-0714 – volume: 4 start-page: 30 year: 2006 end-page: 35 ident: CR7 article-title: The genotyping of the West Nile virus in birds in the far eastern region of Russia in 2002–2004 publication-title: Mol. Gen. Mikrobiol. Virusol. – volume: 13 start-page: 1636 year: 1995 ident: 88777_CR40 publication-title: Vaccine doi: 10.1016/0264-410X(95)00133-L – volume: 32 start-page: 398 year: 2012 ident: 88777_CR2 publication-title: Neuropathology doi: 10.1111/j.1440-1789.2011.01275.x – volume: 72 start-page: 369 year: 2010 ident: 88777_CR10 publication-title: J. Vet. Med. Sci. doi: 10.1292/jvms.09-0311 – volume: 243 start-page: 69 year: 2018 ident: 88777_CR31 publication-title: Virus Res. doi: 10.1016/j.virusres.2017.10.015 – volume: 80 start-page: 9557 year: 2006 ident: 88777_CR37 publication-title: J. Virol. doi: 10.1128/JVI.00080-06 – volume: 55 start-page: 231 year: 2000 ident: 88777_CR15 publication-title: Adv. Virus Res. doi: 10.1016/s0065-3527(00)55005-2 – volume: 70 start-page: 4549 year: 1996 ident: 88777_CR29 publication-title: J. Virol. doi: 10.1128/JVI.70.7.4549-4557.1996 – volume: 291 start-page: 6559 year: 2016 ident: 88777_CR50 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M115.712760 – volume: 25 start-page: 635 year: 2012 ident: 88777_CR14 publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.00045-12 – volume: 16 start-page: e1008238 year: 2020 ident: 88777_CR51 publication-title: PLoS Pathog. doi: 10.1371/journal.ppat.1008238 – volume: 2015 start-page: 376230 year: 2015 ident: 88777_CR3 publication-title: Biomed. Res. Int. doi: 10.1155/2015/376230 – volume: 7 start-page: 723 year: 2016 ident: 88777_CR11 publication-title: Ticks Tick Borne Dis. doi: 10.1016/j.ttbdis.2016.03.002 – volume: 11 start-page: e0005936 year: 2017 ident: 88777_CR38 publication-title: PLoS Negl. Trop. Dis. doi: 10.1371/journal.pntd.0005936 – volume: 22 start-page: 4 year: 2000 ident: 88777_CR41 publication-title: Methods doi: 10.1006/meth.2000.1031 – volume: 45 start-page: 299 year: 1992 ident: 88777_CR9 publication-title: World Health Stat. Q. – volume: 10 start-page: 907 year: 2003 ident: 88777_CR19 publication-title: Nat. Struct. Biol. doi: 10.1038/nsb990 – volume: 180 start-page: 294 year: 1991 ident: 88777_CR46 publication-title: Virology doi: 10.1016/0042-6822(91)90034-9 – volume: 8 start-page: 14722 year: 2017 ident: 88777_CR35 publication-title: Nat. Commun. doi: 10.1038/ncomms14722 – volume: 4 start-page: 1209 year: 2002 ident: 88777_CR39 publication-title: Microbes Infect. doi: 10.1016/S1286-4579(02)01647-7 – volume: 52 start-page: 814 year: 2014 ident: 88777_CR30 publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.02488-13 – volume: 84 start-page: 461 year: 2011 ident: 88777_CR6 publication-title: Am. J. Trop. Med. Hyg. doi: 10.4269/ajtmh.2011.09-0714 – volume: 114 start-page: e180332 year: 2019 ident: 88777_CR4 publication-title: Mem. Inst. Oswaldo Cruz doi: 10.1590/0074-02760180332 – volume: 10 start-page: 7168 year: 2020 ident: 88777_CR28 publication-title: Sci. Rep. doi: 10.1038/s41598-020-64199-7 – volume: 80 start-page: 12149 year: 2006 ident: 88777_CR18 publication-title: J. Virol. doi: 10.1128/JVI.01732-06 – volume: 61 start-page: 3 year: 2003 ident: 88777_CR13 publication-title: Adv. Virus Res. doi: 10.1016/s0065-3527(03)61001-8 – volume: 1 start-page: 5 year: 2003 ident: 88777_CR8 publication-title: Clin. Med. Res. doi: 10.3121/cmr.1.1.5 – volume: 375 start-page: 291 year: 1995 ident: 88777_CR17 publication-title: Nature doi: 10.1038/375291a0 – volume: 73 start-page: 5605 year: 1999 ident: 88777_CR24 publication-title: J. Virol. doi: 10.1128/JVI.73.7.5605-5612.1999 – volume: 107 start-page: 18950 year: 2010 ident: 88777_CR34 publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.1011036107 – volume: 85 start-page: 3637 year: 2004 ident: 88777_CR49 publication-title: J. Gen. Virol. doi: 10.1099/vir.0.80247-0 – volume: 7 start-page: 10047 year: 2017 ident: 88777_CR22 publication-title: Sci. Rep. doi: 10.1038/s41598-017-10595-5 – volume: 7 start-page: 128 year: 2001 ident: 88777_CR5 publication-title: Emerg. Infect. Dis. doi: 10.3201/eid0701.010118 – volume: 69 start-page: 5816 year: 1995 ident: 88777_CR27 publication-title: J. Virol. doi: 10.1128/JVI.69.9.5816-5820.1995 – volume: 11 start-page: 3112 year: 2020 ident: 88777_CR36 publication-title: Nat. Commun. doi: 10.1038/s41467-020-16925-y – volume: 77 start-page: 813 year: 2003 ident: 88777_CR43 publication-title: J. Virol. doi: 10.1128/jvi.77.2.813-820.2003 – volume: 38 start-page: 1823 year: 2000 ident: 88777_CR12 publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.38.5.1823-1826.2000 – volume: 87 start-page: 9933 year: 2013 ident: 88777_CR26 publication-title: J. Virol. doi: 10.1128/JVI.01283-13 – volume: 9 start-page: e100641 year: 2014 ident: 88777_CR42 publication-title: PLoS ONE doi: 10.1371/journal.pone.0100641 – volume: 77 start-page: 11357 year: 2003 ident: 88777_CR44 publication-title: J. Virol. doi: 10.1128/jvi.77.21.11357-11366.2003 – volume: 12 start-page: 279 year: 1994 ident: 88777_CR45 publication-title: Vaccine doi: 10.1016/0264-410x(94)90206-2 – volume: 4 start-page: 30 year: 2006 ident: 88777_CR7 publication-title: Mol. Gen. Mikrobiol. Virusol. – volume: 79 start-page: 13262 year: 2005 ident: 88777_CR32 publication-title: J. Virol. doi: 10.1128/JVI.79.21.13262-13274.2005 – volume: 187 start-page: 290 year: 1992 ident: 88777_CR47 publication-title: Virology doi: 10.1016/0042-6822(92)90317-i – volume: 42 start-page: 4101 year: 2004 ident: 88777_CR21 publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.42.9.4101-4110.2004 – volume: 85 start-page: 4377 year: 2011 ident: 88777_CR25 publication-title: J. Virol. doi: 10.1128/JVI.02458-10 – volume: 70 start-page: 37 issue: Pt 1 year: 1989 ident: 88777_CR1 publication-title: J. Gen. Virol. doi: 10.1099/0022-1317-70-1-37 – volume: 78 start-page: 12591 year: 2004 ident: 88777_CR23 publication-title: J. Virol. doi: 10.1128/JVI.78.22.12591-12602.2004 – volume: 6 start-page: 13 year: 2013 ident: 88777_CR16 publication-title: Viruses doi: 10.3390/v6010013 – volume: 38 start-page: 613 year: 2010 ident: 88777_CR20 publication-title: Biologicals doi: 10.1016/j.biologicals.2010.07.004 – volume: 6 start-page: 6341 year: 2015 ident: 88777_CR33 publication-title: Nat. Commun. doi: 10.1038/ncomms7341 – volume: 61 start-page: 185 year: 2003 ident: 88777_CR48 publication-title: Adv. Virus Res. doi: 10.1016/s0065-3527(03)61005-5 |
| SSID | ssj0000529419 |
| Score | 2.358369 |
| Snippet | West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because... Abstract West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because... |
| SourceID | doaj pubmedcentral proquest pubmed crossref springer |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 9213 |
| SubjectTerms | 631/326/596/1879 631/326/596/2562 Antigenicity Cross-reactivity E protein Encephalitis Enzyme-linked immunosorbent assay Horses Humanities and Social Sciences multidisciplinary Neutralization PrM protein Proteins Science Science (multidisciplinary) Vector-borne diseases Viruses West Nile virus |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LT-MwELZWSEhc0PIOL3klbqxFYjuxfSyoCFa7XHaRuFl24uwiVSmiLRL_npk4KS3s47LH1m7lzMPzOZ75hpCTXNWw5QnFFOABJrO6YtpxwXRpXJCZU6nH9x3fboqrW_nlLr9baPWFOWGRHjgK7gw5z0rNlXLay6CD1sZnvCjB8EyQsXQPYt7CYSqyenMjM9NVyaRCn00gUmE1Gc-YRg48li9Fopaw_3co832y5Jsb0zYQXX4k6x2CpIO48g3yITSbZDX2lHzeIqOFNCA6rqmjyEg8eqZYU4l5QRSMblzFDDv4Czr8ev19QAG7UuwyQ29gm6BP94-zCe0TtRqK2fE_6WTmMSV4RB_6dLptcns5_HFxxbqWCqwEKDZlRlZCuqwGFCSU9LX2ugLHc6UOzquQg3_KILySeYBPZdC1r7wweepTo0IRxA5ZacZN2CMUcFMOhyHjU0AkCk55DqCfq2TBawAhPE1I1ovXlh3fOLa9GNn23ltoG1ViQSW2VYnNE3I6_81DZNv46-xz1Np8JjJlt1-A_dhOEPZf9pOQw17ntnPficVyXgSSGQx_mg-D4-FtimvCeBbnYJWuLBKyG01kvhIhMIYInhC1ZDxLS10eae5_teTeyPNZGHi2z72ZvS7rz6LY_x-iOCBrHP0jlYzrQ7IyfZyFI4BcU3_cetcL3iUj8Q priority: 102 providerName: Directory of Open Access Journals – databaseName: ProQuest Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB5BERIXxJtAQUbiBlYT24ntEyqoVUHQC1Tam2UnTqm0SpbNLlL_PTN5leXR4268K8fz8GfPzDcAr3Ndo8uTmmvEA1xldcWNF5Kb0vqoMq_TQPcdX06LkzP1aZEvxgu3bkyrnHxi76irtqQ78gOqsKS9PbPvVj84dY2i6OrYQuMm3CLqMtJqvdDzHQtFsVRmx1qZVJqDDvcrqikTGTfEhMfznf2op-3_F9b8O2Xyj7hpvx0d34O7I45kh4Pg78ON2DyA20NnycuHsPwtGYi1NfOMeImXl4wqKyk7iKHqtdWQZ4d_wY4-f_x6yBDBMuo1w07RWbCfF-ttx6Z0rYZRjvw567aBEoOXbDUl1T2Cs-Ojbx9O-NhYgZcIyDbcqkoqn9WIhaRWoTbBVGh-vjTRBx1ztFIVZdAqj_ipjKYOVZA2T0NqdSyifAx7TdvEp8AQPeV4JLIhRVyi8aznEQD6ShWiRigi0gSyaXldObKOU_OLpeuj39K4QSQOReJ6kbg8gTfzb1YD58a1o9-T1OaRxJfdf9Guz924EI6Y80ojtPYmqGiiMTZkoijRfdmIR9wE9ieZu9GIO3elcgm8mh-j-VFMxTex3Q5jqFZXFQk8GVRknomUtJNIkYDeUZ6dqe4-aS6-9xTfxPZZWHy3t5OaXU3r_0vx7Pq3eA53BGl-qrgw-7C3WW_jC4RUm_Cyt5tfl_Qcsw priority: 102 providerName: ProQuest – databaseName: Springer Nature HAS Fully OA dbid: AAJSJ link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwEB6VVkhcEO-mFGQkbmCR2E7sHBfUqqygl1KpN8tOnFJplVT7QOq_74zzgIWCxHHXzso7D_tz_M1ngLe5bnDKk5prxANcZU3NjROSm6p0QWVOp57ed3w9LU7O1fwiv9gBMdbCRNJ-lLSM0_TIDvuwwoWGisFExg1J2PH8HuyRVDvG9t5sNj-bT29W6OxKZeVQIZNKc8fDW6tQFOu_C2H-SZT87bQ0LkLHj-DhgB7ZrB_vY9gJ7RO4398nefMUFr9QgFjXMMdIjXhxw6iekjhBDAOuq3t2Hf4EO_ry-WzGELcyumGGneIUwX5cLTcrNpK0WkbM-Eu22niiAy_Y9WisZ3B-fPTt0wkfrlPgFcKwNS9VLZXLGkRAUivfGG9qTDpXmeC8DjnmpgrSo00DfqqCaXztZZmnPi11KIJ8Drtt14Z9YIiZctwIlT5FNKJxh-cQ9rlaFaJBACLSBLLRvLYatMbpyouFjWfe0tjeJRZdYqNLbJ7Au-mZ615p45-9P5LXpp6kkh2_6JaXdjCEJb28ygitnfEqmGBM6TNRVDhplQE3tgkcjj63Q-quLJXyEojMsPnN1IxJRycprg3dpu9DFbqqSOBFHyLTSKSk9UOKBPRW8GwNdbulvfoehb1J47Mo8b-9H8Ps57D-boqD_-v-Eh4IyoRUcWEOYXe93IRXCKzW_vWQSbeyPRwC priority: 102 providerName: Springer Nature |
| Title | Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles |
| URI | https://link.springer.com/article/10.1038/s41598-021-88777-5 https://www.ncbi.nlm.nih.gov/pubmed/33911132 https://www.proquest.com/docview/2519143519 https://www.proquest.com/docview/2519811746 https://pubmed.ncbi.nlm.nih.gov/PMC8080695 https://doaj.org/article/0102c8277a8b4e8e889b126c4889e409 |
| Volume | 11 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1bb9MwFLZ2ERIviPvCRmUk3sCQ2E7sPCDUVZ1GxSrEqNQ3y06cMSlKRy8T_feck0uhUJB4iho7kXsu9uf4nO8Q8jJWBUx5QjEFeIDJqMiZtlwwnaXWy8iq0OH3jotxcj6Ro2k83SNduaNWgIudWzusJzWZl2--f1u_B4d_16SM67cLWIQwUYxHTCO9HYv3ySGsTAorGly0cL_h-uaprGt9IAk7AzDB2zya3a_ZWqtqSv9dOPTPcMrfzlTrpersPrnXYkzab4ziAdnz1UNyp6k6uX5Eyl8CheisoJYiZ3G5pph1iZFDFMxyljcxePAKOvz44bJPAd1SrENDxzCR0Nvr-WpBu1CuimL8_BVdrBwGDZf0pgu4e0wmZ8Mvg3PWFl1gGYC1JUtlLqSNCsBJQklXaKdzcE2baW-d8jF4sPTCKRl7-JV5XbjciTQOXZgqn3jxhBxUs8ofEQrIKobtUupCwCwK9oEWwKHNZcILgCk8DEjUiddkLSM5FsYoTX0yLrRpVGJAJaZWiYkD8mrzzE3Dx_HP3qeotU1P5NKub8zmV6YVhEFWvUxzpax20muvdeoinmQwtaUetr8BOel0bjr7NJjwi1AzguYXm2ZwTTxvsZWfrZo-mMcrk4A8bUxkMxIhcJURPCBqy3i2hrrdUl1_rem_kQk0SeG_ve7M7Oew_i6KZ_8luGNyl6MjhJJxfUIOlvOVfw7oa-l6ZF9NVY8c9vujyxFcT4fjT5_h7iAZ9OovGr3a6X4AQYErhg |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwELZKEYIL4lkCBYwEJ7Ca2E7sHBAq0GqXbvdCK-3N2IlTKq2SZbML2j_Fb2Qmjy3Lo7cekziR4xnPfON5EfIyVgWIPKGYAjzAZFTkTFsumM5S62VkVejwvON4nAxO5adJPNkiP_tcGAyr7GViI6jzKsMz8j3MsETdHqXvZt8Ydo1C72rfQqNliyO_-gEmW_12-BHo-4rzw4OTDwPWdRVgGaCRBUtlLqSNCgACQklXaKdz4D2baW-d8jGwqPTCKRl7uMq8LlzuRBqHLkyVT7yA714j10HxhmjsqYlan-mg10xGaZebEwq9V4N-xBw2HjGNlfdYvKH_mjYB_8K2f4do_uGnbdTf4R1yu8OtdL9ltLtky5f3yI22k-XqPpn-FnxEq4JainWQpyuKmZwYjUSB1au8jeuDT9CD0fDzPgXETLG3DR2DcKLfz-fLmvbhYSXFmPwzWi8dBiJP6awP4ntATq9kyR-S7bIq_SNCAa3FYIKlLgQcpMC2tAA4bS4TXgD04WFAon55TdZVOcdmG1PTeNuFNi1JDJDENCQxcUBer9-ZtTU-Lh39Hqm2Hon1uZsb1fzMdAthsFJfprlSVjvptdc6dRFPMhCXqQeTOiC7Pc1NJzRqc8HiAXmxfgzbHX04tvTVsh2DucEyCchOyyLrmQiBmkvwgKgN5tmY6uaT8vxrU1Icq4smKfzbm57NLqb1_6V4fPlfPCc3ByfHIzMajo-ekFscd0EoGde7ZHsxX_qnAOcW7lmzhyj5ctWb9hd1n1qC |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Jb9QwFLbKVCAuiJ1AASPBCaxJbCd2Dgi1dEYtLaMKqNSbaydOqTRKhllA89f4dbyXZcqw9NZjEidy_BZ_z28j5GWsClB5QjEFeIDJqMiZtlwwnaXWy8iq0OF5x8dRsncsP5zEJxvkZ5cLg2GVnU6sFXVeZXhG3scMS9zbo7RftGERR7vDd5NvDDtIoae1a6fRsMiBX_4A8232dn8XaP2K8-Hgy_s91nYYYBkgkzlLZS6kjQoABUJJV2inc-BDm2lvnfIxsKv0wikZe7jKvC5c7kQahy5MlU-8gO9eI5sKraIe2dwZjI4-rU540Icmo7TN1AmF7s9gt8SMNh4xjXX4WLy2G9ZNA_6FdP8O2PzDa1tvhsPb5FaLYul2w3Z3yIYv75LrTV_L5T0y_i0UiVYFtRSrIo-XFPM6MTaJAuNXeRPlB5-gg8P9z9sU8DPFTjd0BKqKfj-fLma0CxYrKUbon9HZwmFY8phOupC---T4Shb9AemVVekfEQrYLQaDLHUhoCIFlqYF-GlzmfACgBAPAxJ1y2uytuY5tt4Ym9r3LrRpSGKAJKYmiYkD8nr1zqSp-HHp6B2k2mokVuuub1TTM9MuhMG6fZnmSlntpNde69RFPMlAeaYeDOyAbHU0N60KmZkLhg_Ii9VjEH706NjSV4tmDGYKyyQgDxsWWc1ECNzHBA-IWmOetamuPynPv9YFxrHWaJLCv73p2OxiWv9fiseX_8VzcgME1hzujw6ekJschSCUjOst0ptPF_4pYLu5e9YKESWnVy23vwCjeGAd |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Development+of+a+highly+specific+serodiagnostic+ELISA+for+West+Nile+virus+infection+using+subviral+particles&rft.jtitle=Scientific+reports&rft.au=Maezono%2C+Keisuke&rft.au=Kobayashi%2C+Shintaro&rft.au=Tabata%2C+Koshiro&rft.au=Yoshii%2C+Kentaro&rft.date=2021-04-28&rft.issn=2045-2322&rft.eissn=2045-2322&rft.volume=11&rft.issue=1&rft_id=info:doi/10.1038%2Fs41598-021-88777-5&rft.externalDBID=n%2Fa&rft.externalDocID=10_1038_s41598_021_88777_5 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon |