Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

• Published in: Nature biotechnology Vol. 33; no. 1; pp. 73 - 80
• Main Authors: Zuris, John A, Thompson, David B, Shu, Yilai, Guilinger, John P, Bessen, Jeffrey L, Hu, Johnny H, Maeder, Morgan L, Joung, J Keith, Chen, Zheng-Yi, Liu, David R
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.01.2015; Nature Publishing Group
• Subjects: 631/1647/1511; 631/61/2297; 631/61/51; Acids; Agriculture; Bioinformatics; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Cations; Genetic aspects; Genomes; Hair cells (Mechanoreceptors); In Vitro Techniques; Life Sciences; Lipids; Lipids - administration & dosage; Nucleic acids; Physiological aspects; Proteins; Proteins - administration & dosage; Reagents; Trans-Activators - administration & dosage; Transcription factors; Transfection; United States

Efficient protein delivery using cationic lipid transfection reagents enables high efficiency protein-based genome editing in vivo and in vitro . Efficient intracellular delivery of proteins is needed to fully realize the potential of protein therapeutics. Current methods of protein delivery commonl...