A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cell Derived Neuro-Progenitors

• Published in: Advanced materials (Weinheim) Vol. 28; no. 1; pp. 161 - 167
• Main Authors: Bouyer, Charlène, Chen, Pu, Güven, Sinan, Demirtaş, Tuğrul Tolga, Nieland, Thomas J. F., Padilla, Frédéric, Demirci, Utkan
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.01.2016; Wiley Subscription Services, Inc
• Subjects: Acoustic radiation; Acoustics; Assembly; Brain - cytology; bulk acoustic standing waves; Cell Differentiation; Construction; Elongation; Fibrin; Human; Human Embryonic Stem Cells - cytology; Humans; multilayered 3D tissue structures; neural constructs; neural progenitor cells; Neural Stem Cells - cytology; Sound waves; Stem cells; Three dimensional; tissue engineering; Tissue Engineering - methods; bulk acoustic standing waves; multilayered 3D tissue structures; tissue engineering; neural progenitor cells; neural constructs
• ISSN: 0935-9648; 1521-4095
• DOI: 10.1002/adma.201503916

A bio‐acoustic levitational assembly method for engineering of multilayered, 3D brainlike constructs is presented. Acoustic radiation forces are used to levitate neuroprogenitors derived from human embryonic stem cells in 3D multilayered fibrin tissue constructs. The neuro‐progenitor cells are subsequently differentiated in neural cells, resulting in a 3D neuronal construct with inter and intralayer neurite elongations.