Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability

• Published in: Animal models and experimental medicine Vol. 7; no. 6; pp. 824 - 834
• Main Authors: Zhang, Yao, Xu, Chang, Huang, Yun, Tan, Dongmei, Luo, Wenping, Zhang, Yan, Tan, Yi
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.12.2024; Laboratory of Animal Center,Chongqing Medical University,Chongqing,China%Laboratory of Animal Center,Southwest University,Chongqing,China%Stomatological Hospital of Chongqing Medical University,Chongqing,China; John Wiley and Sons Inc; Wiley
• Subjects: alizarin; Alkaline phosphatase; Animals; antigens; bone formation; Bone growth; Bone healing; Bone marrow; Bone Marrow Cells - cytology; bone marrow mesenchymal stem cells; Bone morphogenetic protein 9; bone morphogenetic proteins; calcium; Carbon dioxide; Cell culture; Cell cycle; Cell Differentiation; cell lines; Computed tomography; Flow cytometry; GelMA hydrogel; Growth Differentiation Factor 2; histology; Hydrogels; immortalization; Laboratory animals; medicine; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; micro-computed tomography; New Zealand; Original; Ossification (ectopic); Osteogenesis; Plasmids; Rabbits; Stem cells; Themed Section: Bone Regeneration and Disease Mechanisms; Tissue engineering; Tissue Engineering - methods; Tumorigenesis; Variance analysis; New Zealand; Beijing China; United States > US; China; GelMA hydrogel; bone morphogenetic protein 9; bone marrow mesenchymal stem cells; immortalization

Background A stable and standardized source of mesenchymal stem cells is a prerequisite for bone repair tissue engineering research and application. We aimed to establish a stable cell line of bone marrow mesenchymal stem cells from New Zealand rabbits and explore their osteogenic differentiation ca...