Microfluidic immunoassays as rapid saliva-based clinical diagnostics

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 104; no. 13; pp. 5268 - 5273
• Main Authors: Herr, Amy E, Hatch, Anson V, Throckmorton, Daniel J, Tran, Huu M, Brennan, James S, Giannobile, William V, Singh, Anup K
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 27.03.2007; National Acad Sciences
• Subjects: Anatomy & physiology; Antibodies; Bioassays; Biological markers; Biological Sciences; biomarkers; Biomarkers - metabolism; Body fluids; Chemistry, Clinical - methods; Collagen - metabolism; Computers, Handheld; diagnostic techniques; Diagnostic tests; Disease; disease course; Disease progression; electrophoresis; Electrophoresis - methods; Enzyme-Linked Immunosorbent Assay; Enzymes; Equipment Design; Gels; health status; Human subjects; Humans; Image Processing, Computer-Assisted; Immunoassay; Immunoassay - methods; Immunoassays; Matrix Metalloproteinase 8 - metabolism; Microfluidics - methods; mixing; Mouth diseases; P branes; Periodontal diseases; Periodontal Diseases - diagnosis; Periodontal Diseases - metabolism; Periodontics; Physical Sciences; pregnancy; Saliva; Saliva - metabolism; Ultraviolet Rays

At present, point-of-care (POC) diagnostics typically provide a binary indication of health status (e.g., home pregnancy test strip). Before anticipatory use of diagnostics for assessment of complex diseases becomes widespread, development of sophisticated bioassays capable of quantitatively measuri...