Morphologic characteristics of the placental basal plate in in vitro fertilization pregnancies: a possible association with the amount of bleeding in delivery

• Published in: Human pathology Vol. 46; no. 8; pp. 1171 - 1179
• Main Authors: Nakamura, Yuki, MD, Yaguchi, Chizuko, MD, Itoh, Hiroaki, MD, DMedSci, Sakamoto, Ryoko, BS, Kimura, Takako, CT, Furuta, Naomi, MD, Uchida, Toshiyuki, MD, PhD, Tamura, Naoaki, MD, PhD, Suzuki, Kazunao, MD, PhD, Sumimoto, Kazuhiro, PhD, Matsuda, Yumiko, CT, Matsuura, Toshiki, MD, PhD, Nishimura, Mitsuru, MD, PhD, Kanayama, Naohiro, MD, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2015; Elsevier Limited
• Subjects: Assisted reproductive technology (ART); Cesarean section; Childbirth & labor; Delivery; Delivery, Obstetric - adverse effects; Embryos; Female; Fertilization in Vitro - adverse effects; Hemorrhage - etiology; Hemorrhage - pathology; Humans; Immunohistochemistry; In vitro fertilization; Morphology; Pathology; Placenta; Placenta - pathology; Postpartum hemorrhage (PPH); Pregnancy; Studies; Pregnancy; Postpartum hemorrhage (PPH); Placenta; Delivery; Assisted reproductive technology (ART)
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2015.04.007

Summary The aim of the present study was to investigate the relationship between assisted reproductive technology procedures, the morphology of the basal plate of placentas, and amount of bleeding in deliveries. Fifty-five whole placentas (fresh-embryo transfer in the in vitro fertilization cycle [n = 6], frozen-thawed embryo transfer in the natural cycle [n = 13] or in the hormonal cycle [n = 10], and age-matched spontaneously conceived pregnancies [n = 26]) were retrospectively enrolled and histologically analyzed. The whole placentas were stored in our pathological division among 512 singleton pregnancies with vaginal deliveries (34-41 weeks of gestation) at Hamamatsu University Hospital. The morphology of the placental basal plate was examined using Azan staining. A total of 20 digital images (each 0.53 mm2 ) of microscopic fields were analyzed per placenta to measure the mean values of the vertical maximum thickness of Rohr and Nitabuch fibrinoid layers and % loss of decidua. The thickness of Rohr fibrinoid layer and % loss of decidua were significantly higher in the frozen-thawed embryo transfer in the hormonal cycle group than in the frozen-thawed embryo transfer in the natural cycle and spontaneously conceived pregnancy groups (each P < .01). The z scores for both the thickness of Rohr fibrinoid layer and % loss of decidua positively correlated with those for the amount of bleeding in deliveries ( P < .05 each). Assisted reproductive technology procedures changed the morphology of the placental basal plate, suggesting a possible association with an increase in the amount of bleeding in deliveries.