Systematic evaluation of suspension MDCK cells, adherent MDCK cells, and LLC-MK2 cells for preparing influenza vaccine seed virus

• Published in: Vaccine Vol. 37; no. 43; pp. 6526 - 6534
• Main Authors: Nakamura, Kazuya, Harada, Yuichi, Takahashi, Hitoshi, Trusheim, Heidi, Bernhard, Roth, Hamamoto, Itsuki, Hirata-Saito, Asumi, Ogane, Teruko, Mizuta, Katsumi, Konomi, Nami, Konomi, Yasushi, Asanuma, Hideki, Odagiri, Takato, Tashiro, Masato, Yamamoto, Norio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 08.10.2019; Elsevier Limited
• Subjects: Allergy and Immunology; Amino acid substitution; Amino acids; Antigenicity; Antigens; Binding sites; dogs; Eggs; Exo-a-sialidase; genes; Genetic and antigenic stability; genetic stability; Glycosylation; HA protein; Health care; hemagglutination; Hemagglutination inhibition; Hemagglutinins; hens; humans; Illnesses; Infections; Influenza; Influenza A virus; Influenza vaccine seed virus; influenza vaccines; Kidneys; Macaca mulatta; Madin–Darby canine kidney cell line; Mutation; Pandemics; sialidase; Stability; Suspension culture; Vaccines; virus replication; Viruses; United States > US; Japan; HI; Suspension culture; MDCK-N; NA; MDCK cells; EID50; Madin–Darby canine kidney cell line; MDCK-C; Influenza vaccine seed virus; Genetic and antigenic stability; HA; LLC-MK2D; adherent MDCK cells; hemagglutination inhibition; 50% egg infectious dose; hemagglutinin; Madin–Darby canine kidney cells; adherent LLC-MK2 cells; neuraminidase; suspension MDCK cells; EID 50

Suspension Madin–Darby canine kidney (MDCK) cells (MDCK-N), adherent MDCK cells (MDCK-C), and adherent rhesus monkey kidney LLC-MK2 cells (LLC-MK2D) were systematically evaluated for the preparation of influenza vaccine seed viruses for humans on the basis of primary virus isolation efficiency, grow...