Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 104; no. 8; pp. 2614 - 2619
• Main Authors: Hsu, Tsui-Ling, Hanson, Sarah R, Kishikawa, Kuniyuki, Wang, Sheng-Kai, Sawa, Masaaki, Wong, Chi-Huey
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 20.02.2007; National Acad Sciences
• Subjects: alkynes; Alkynes - chemistry; Alkynes - metabolism; Azides - chemistry; Biochemistry; Biological Sciences; Biotinylation; Cancer; Carbohydrates; Cell Extracts; Cell Line, Tumor; Cell lines; Cells; copper; Cytometry; engineering; Fluorescence; Fluorescent Dyes - metabolism; fucose; Fucose - chemistry; Fucose - metabolism; Glycoconjugates; Glycoconjugates - metabolism; Glycoproteins; glycosylation; Hexosamines - chemistry; Hexosamines - metabolism; Humans; image analysis; Jurkat Cells; Lectins; Microscopy; Molecular Probes - metabolism; N-Acetylneuraminic Acid - chemistry; neoplasms; oligosaccharides; Polysaccharides; sialic acids; Sugar; Sugars; T lymphocytes

Developing tools for investigating the cellular activity of glycans will help to delineate the molecular basis for aberrant glycosylation in pathological processes such as cancer. Metabolic oligosaccharide engineering, which inserts sugar-reporting groups into cellular glycoconjugates, represents a...