Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

• Published in: Nature (London) Vol. 582; no. 7813; pp. 561 - 565
• Main Authors: Thi Nhu Thao, Tran, Labroussaa, Fabien, Ebert, Nadine, V’kovski, Philip, Stalder, Hanspeter, Portmann, Jasmine, Kelly, Jenna, Steiner, Silvio, Holwerda, Melle, Kratzel, Annika, Gultom, Mitra, Schmied, Kimberly, Laloli, Laura, Hüsser, Linda, Wider, Manon, Pfaender, Stephanie, Hirt, Dagny, Cippà, Valentina, Crespo-Pomar, Silvia, Schröder, Simon, Muth, Doreen, Niemeyer, Daniela, Corman, Victor M., Müller, Marcel A., Drosten, Christian, Dijkman, Ronald, Jores, Joerg, Thiel, Volker
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.06.2020; Nature Publishing Group
• Subjects: 42/70; 45/91; 631/326/596; 631/326/596/2078; 631/326/596/4130; Analysis; Animals; Artificial chromosomes; Betacoronavirus - genetics; China - epidemiology; Chlorocebus aethiops; Chromosomes; Chromosomes, Artificial, Yeast - metabolism; Cloning; Cloning, Molecular - methods; Coronaviridae; Coronavirus Infections - epidemiology; Coronavirus Infections - virology; Coronaviruses; COVID-19; Deoxyribonucleic acid; DNA; DNA-directed RNA polymerase; DNA-Directed RNA Polymerases - metabolism; E coli; Epidemics; Evolution, Molecular; Flaviviridae; Fragments; Genetic aspects; Genetic engineering; Genetically modified organisms; Genetics; Genome, Viral - genetics; Genomes; Genomic analysis; Genomics; Genomics - methods; Humanities and Social Sciences; Humans; Methods; Middle East respiratory syndrome; multidisciplinary; Mutation; Pandemics; Pandemics - statistics & numerical data; Pathogenesis; Pneumonia, Viral - epidemiology; Pneumonia, Viral - virology; Pneumoviridae; Recombination; Respiratory diseases; Respiratory Syncytial Viruses - genetics; Reverse engineering; Reverse Genetics - methods; Ribonucleic acid; RNA; RNA polymerase; RNA viruses; Saccharomyces cerevisiae - genetics; SARS-CoV-2; Science; Science (multidisciplinary); Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Synthetic Biology - methods; Vaccine development; Vaccines; Vero Cells; Viral diseases; Viral Proteins - metabolism; Viruses; Yeast; Yeasts; Zika virus; Zika Virus - genetics; China; Switzerland

Reverse genetics has been an indispensable tool to gain insights into viral pathogenesis and vaccine development. The genomes of large RNA viruses, such as those from coronaviruses, are cumbersome to clone and manipulate in Escherichia coli owing to the size and occasional instability of the genome...