Engineered Biosynthesis of a Complete Macrolactone in a Heterologous Host - Kao, Camilla M.; Katz, Leonard; Khosla, Chaitan (Science (American Association for the Advancement of Science), 1994)
Keywords: Acetates; Active sites; Acyl Coenzyme A - metabolism; Base Sequence; Binding Sites; Biochemistry; Bioconversions. Hemisynthesis; Biological and medical sciences; Biosynthesis; Biotechnology; Chemical engineering; Cloning, Molecular; Drug Design; Enzymes; Erythromycin - analogs & derivatives; Erythromycin - biosynthesis; Erythromycin - isolation & purification; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetic Engineering; Genetic Vectors; Mass spectroscopy; Methods. Procedures. Technologies; Molecular Sequence Data; Multidisciplinary Sciences; Multienzyme Complexes - chemistry; Multienzyme Complexes - genetics; Multienzyme Complexes - metabolism; Multigene Family; Mutation; Oleandomycin - analogs & derivatives; Oleandomycin - biosynthesis; Oleandomycin - isolation & purification; Plasmids; Polyketides; Recombinant Proteins - metabolism; Saccharopolyspora erythraea; Science & Technology; Science & Technology - Other Topics; Streptomyces - enzymology; Streptomyces - genetics; Streptomyces coelicolor; Structure-Activity Relationship; PHYSICOCHEMICAL PROPERTIES; PROPIONIBACTERIUM-SHERMANII; POLYKETIDE SYNTHASE; ESCHERICHIA-COLI; COENZYME-A MUTASE; SACCHAROPOLYSPORA-ERYTHRAEA; MACROLIDE ANTIBIOTICS; METHYLMALONYL-COA EPIMERASE; STREPTOMYCES-ERYTHREUS; NUCLEOTIDE-SEQUENCE; Actinomycetales; Heterologous system; Streptomycetaceae; Genetic transformation; Streptomyces coelicolor; Aglycone; Bacteria; Actinomycetes; Biosynthesis; Recombinant DNA; Erythromycin; Macrolide