Detection of genes involved in biodegradation and biotransformation in microbial communities by using 50-mer oligonucleotide microarrays

• Published in: Applied and Environmental Microbiology Vol. 70; no. 7; pp. 4303 - 4317
• Main Authors: Rhee, S.K, Liu, X, Wu, L, Chong, S.C, Wan, X, Zhou, J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.07.2004
• Subjects: Bacteria - genetics; benzene; Biodegradation; Biodegradation, Environmental; Biological and medical sciences; bioremediation; Biotransformation; Burkholderia; Comamonas; DNA probes; ethyl benzene; Fundamental and applied biological sciences. Psychology; gene expression; genes; Hybridization; Methods; microarray technology; Microbiology; naphthalene; Naphthalenes - metabolism; Oligonucleotide Array Sequence Analysis - methods; polluted soils; polycyclic aromatic hydrocarbons; Polymerase Chain Reaction; Pseudomonas; Quantitative genetics; Ralstonia; Rhodococcus; Sensitivity and Specificity; soil bacteria; Soil contamination; Soil Microbiology; Soil microorganisms; soil pollution; Test methods; toluene; xylene; Biodegradation; Analysis method; Gene; Biotransformation; Oligonucleotide; Detection; Microbial community

To effectively monitor biodegrading populations, a comprehensive 50-mer-based oligonucleotide microarray was developed based on most of the 2,402 known genes and pathways involved in biodegradation and metal resistance. This array contained 1,662 unique and group-specific probes with <85% similar...