Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology

• Published in: Cell Vol. 183; no. 4; pp. 1024 - 1042.e21
• Main Authors: Piccoli, Luca, Park, Young-Jun, Tortorici, M. Alejandra, Czudnochowski, Nadine, Walls, Alexandra C., Beltramello, Martina, Silacci-Fregni, Chiara, Pinto, Dora, Rosen, Laura E., Bowen, John E., Acton, Oliver J., Jaconi, Stefano, Guarino, Barbara, Minola, Andrea, Zatta, Fabrizia, Sprugasci, Nicole, Bassi, Jessica, Peter, Alessia, De Marco, Anna, Nix, Jay C., Mele, Federico, Jovic, Sandra, Rodriguez, Blanca Fernandez, Gupta, Sneha V., Jin, Feng, Piumatti, Giovanni, Lo Presti, Giorgia, Pellanda, Alessandra Franzetti, Biggiogero, Maira, Tarkowski, Maciej, Pizzuto, Matteo S., Cameroni, Elisabetta, Havenar-Daughton, Colin, Smithey, Megan, Hong, David, Lepori, Valentino, Albanese, Emiliano, Ceschi, Alessandro, Bernasconi, Enos, Elzi, Luigia, Ferrari, Paolo, Garzoni, Christian, Riva, Agostino, Snell, Gyorgy, Sallusto, Federica, Fink, Katja, Virgin, Herbert W., Lanzavecchia, Antonio, Corti, Davide, Veesler, David
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.11.2020; Elsevier
• Subjects: Angiotensin-Converting Enzyme 2; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - blood; Antibodies, Neutralizing - chemistry; Antibodies, Neutralizing - immunology; Antibodies, Viral - blood; Antibodies, Viral - chemistry; Antibodies, Viral - immunology; Antigen-Antibody Reactions; BASIC BIOLOGICAL SCIENCES; Betacoronavirus - immunology; Betacoronavirus - isolation & purification; Betacoronavirus - metabolism; Binding Sites; Biochemistry, Molecular Biology; blood serum; Coronavirus Infections - pathology; Coronavirus Infections - virology; coronaviruses; COVID-19; COVID-19 infection; effector functions; Epitope Mapping - methods; Epitopes - chemistry; Epitopes - immunology; half life; Humans; immunity; Immunoglobulin A - blood; Immunoglobulin A - immunology; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunoglobulin M - blood; Immunoglobulin M - immunology; Kinetics; Life Sciences; Molecular Dynamics Simulation; neutralizing antibodies; nucleoproteins; Pandemics; Peptidyl-Dipeptidase A - chemistry; Peptidyl-Dipeptidase A - metabolism; Pneumonia, Viral - pathology; Pneumonia, Viral - virology; Protein Binding; Protein Domains - immunology; Protein Structure, Quaternary; SARS-CoV-2; serology; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; Spike Glycoprotein, Coronavirus - metabolism; therapeutics; vaccine development; COVID-19; neutralizing antibodies; SARS-CoV-2; coronaviruses; immunity; effector functions

Analysis of the specificity and kinetics of neutralizing antibodies (nAbs) elicited by SARS-CoV-2 infection is crucial for understanding immune protection and identifying targets for vaccine design. In a cohort of 647 SARS-CoV-2-infected subjects, we found that both the magnitude of Ab responses to...