Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells

Background： Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 （MMP- 13）, a member of the collagenase subfamily, is involved in many diff...

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Published inChinese medical journal Vol. 130; no. 6; pp. 717 - 721
Main Authors Li, Xiao-Dong, Zhang, Xin-Rui, Li, Zhi-Hao, Yang, Yang, Zhang, Duo, Zheng, Heng, Dong, Shu-Ying, Chen, Juan, Zeng, Xian-Dong
Format Journal Article
LanguageEnglish
Published China Medknow Publications and Media Pvt. Ltd 20.03.2017
Lippincott Williams & Wilkins Ovid Technologies
Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China%Department of Dermatology Key Laboratory of Immunodermatology, No.1 Hospital of China Medical University, Shenyang, Liaoning 110001, China%Department of Oncology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China
Medknow Publications & Media Pvt Ltd
Wolters Kluwer
Subjects
Animals
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Apoptosis; Dendritic Cell; Matrix Metallopeptidase 13; Maturation; Phagocytosis
Bone marrow
Bone Marrow Cells - cytology
DCS控制系统
Dendritic cells
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - metabolism
Dermatology
Experiments
Female
Flow cytometry
Gene expression
Health aspects
Hospitals
Immunoglobulins
Inflammation
Lipopolysaccharides - pharmacology
Matrix Metalloproteinase 13 - metabolism
Matrix Metalloproteinase 13 - physiology
Matrix metalloproteinase inhibitors
Medical research
Mice
Mice, Inbred C57BL
Original
Polymerase chain reaction
Proteins
RNA, Small Interfering
Western印迹法
凋亡相关基因
基质金属蛋白酶
小鼠骨髓
抗原呈递细胞
树突状细胞
细胞功能
United States > US
Matrix Metallopeptidase 13
Maturation
Phagocytosis
Dendritic Cell
Apoptosis
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Abstract Background： Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 （MMP- 13）, a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells （DCs）. The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis. Methods： Bone marrow-derived dendritic cells were obtained fiom C57BL/6 mice. One short-interfering RNA specific for MMP- 13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry. Results： Compared to the control DCs, MMP- 13-silenced DCs increased expression of anti-apoptosis-related genes, BAGl （control group vs. MMP-13-silenced group： 4.08 ± 0.60 vs. 6.11 ± 0.87, P = 0.008）, BCL-2 （control group vs. MMP-13-silenced group： 7.54 ± 0.76 vs. 9.54 ± 1.29, P = 0.036）, and TP73 （control group vs. MMP- 13-silenced group： 4.33 ± 0.29 vs. 5.60 ±0.32, P = 0.001 ） and decreased apoptosis-related genes, CASPI （control group vs. MMP- 13-silenced group： 3.79±0.67 vs. 2.54±0.39, P - 0.019）, LTBR （control group vs. MMP- 13-silenced group： 9.23 ±1.25 vs. 6.24 ± 1.15, P = 0.012）, and CASP4 （control group vs. MMP-13-silenced group： 2.07 ± 0.56 vs. 0.35 ±0.35, P = 0.002）. Protein levels confirmed the same expression pattern. MMP- 13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability. Conclusion： These results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
AbstractList Background:Dendritic cells are professional antigen-presenting ceils found in an immature state in epithelia and interstitial space,where they capture antigens such as pathogens or damaged tissue.Matrix metallopeptidase 13 (MMP-13),a member of the collagenase subfamily,is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs).The function of MMP-13 in DCs is not well understood.Here,we investigated the effect of MMP-13 on DC maturation,apoptosis,and phagocytosis.Methods:Bone marrow-derived dendritic cells were obtained from C57BL/6 mice.One short-interfering RNA specific for MMP-13 was used to transfect DCs.MMP-13-silenced DCs and control DCs were prepared,and apoptosis was measured using real-time polymerase chain reaction and Western blotting.MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry.Results:Compared to the control DCs,MMP-13-silenced DCs increased expression ofanti-apoptosis-related genes,BAG1 (control group vs.MMP-13-silenced group:4.08 ± 0.60 vs.6.11 ± 0.87,P =0.008),BCL-2 (control group vs.MMP-13-silenced group:7.54 ± 0.76 vs.9.54 ±1.29,P =0.036),and TP73 (control group vs.MMP-13-silenced group:4.33 ± 0.29 vs.5.60 ± 0.32,P =0.001) and decreased apoptosis-related genes,CASP1 (control group vs.MMP-13-silenced group:3.79 ± 0.67 vs.2.54 ± 0.39,P =0.019),LTBR (control group vs.MMP-13-silenced group:9.23 ± 1.25 vs.6.24 ± 1.15,P =0.012),and CASP4 (control group vs.MMP-13-silenced group:2.07 ± 0.56 vs.0.35 ± 0.35,P =0.002).Protein levels confirmed the same expression pattern.MMP-13-silenced groups decreased expression of CD86 on DCs;however,there was no statistical difference in CD80 surface expression.Furthermore,MMP-13-silenced groups exhibited weaker phagocytosis capability.Conclusion:These results indicate that MMP-13 inhibition dampens DC maturation,apoptosis,and phagocytosis.
BACKGROUNDDendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 (MMP-13), a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs). The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis.METHODSBone marrow-derived dendritic cells were obtained from C57BL/6 mice. One short-interfering RNA specific for MMP-13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry.RESULTSCompared to the control DCs, MMP-13-silenced DCs increased expression of anti-apoptosis-related genes, BAG1 (control group vs. MMP-13-silenced group: 4.08 ± 0.60 vs. 6.11 ± 0.87, P = 0.008), BCL-2 (control group vs. MMP-13-silenced group: 7.54 ± 0.76 vs. 9.54 ± 1.29, P = 0.036), and TP73 (control group vs. MMP-13-silenced group: 4.33 ± 0.29 vs. 5.60 ± 0.32, P = 0.001) and decreased apoptosis-related genes, CASP1 (control group vs. MMP-13-silenced group: 3.79 ± 0.67 vs. 2.54 ± 0.39, P = 0.019), LTBR (control group vs. MMP-13-silenced group: 9.23 ± 1.25 vs. 6.24 ± 1.15, P = 0.012), and CASP4 (control group vs. MMP-13-silenced group: 2.07 ± 0.56 vs. 0.35 ± 0.35, P = 0.002). Protein levels confirmed the same expression pattern. MMP-13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability.CONCLUSIONThese results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
Background： Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 （MMP- 13）, a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells （DCs）. The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis. Methods： Bone marrow-derived dendritic cells were obtained fiom C57BL/6 mice. One short-interfering RNA specific for MMP- 13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry. Results： Compared to the control DCs, MMP- 13-silenced DCs increased expression of anti-apoptosis-related genes, BAGl （control group vs. MMP-13-silenced group： 4.08 ± 0.60 vs. 6.11 ± 0.87, P = 0.008）, BCL-2 （control group vs. MMP-13-silenced group： 7.54 ± 0.76 vs. 9.54 ± 1.29, P = 0.036）, and TP73 （control group vs. MMP- 13-silenced group： 4.33 ± 0.29 vs. 5.60 ±0.32, P = 0.001 ） and decreased apoptosis-related genes, CASPI （control group vs. MMP- 13-silenced group： 3.79±0.67 vs. 2.54±0.39, P - 0.019）, LTBR （control group vs. MMP- 13-silenced group： 9.23 ±1.25 vs. 6.24 ± 1.15, P = 0.012）, and CASP4 （control group vs. MMP-13-silenced group： 2.07 ± 0.56 vs. 0.35 ±0.35, P = 0.002）. Protein levels confirmed the same expression pattern. MMP- 13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability. Conclusion： These results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
Background: Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 (MMP-13), a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs). The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis. Methods: Bone marrow-derived dendritic cells were obtained from C57BL/6 mice. One short-interfering RNA specific for MMP-13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry. Results: Compared to the control DCs, MMP-13-silenced DCs increased expression of anti-apoptosis-related genes, BAG1 (control group vs. MMP-13-silenced group: 4.08 ± 0.60 vs. 6.11 ± 0.87, P = 0.008), BCL-2 (control group vs. MMP-13-silenced group: 7.54 ± 0.76 vs. 9.54 ± 1.29, P = 0.036), and TP73 (control group vs. MMP-13-silenced group: 4.33 ± 0.29 vs. 5.60 ± 0.32, P = 0.001) and decreased apoptosis-related genes, CASP1 (control group vs. MMP-13-silenced group: 3.79 ± 0.67 vs. 2.54 ± 0.39, P = 0.019), LTBR (control group vs. MMP-13-silenced group: 9.23 ± 1.25 vs. 6.24 ± 1.15, P = 0.012), and CASP4 (control group vs. MMP-13-silenced group: 2.07 ± 0.56 vs. 0.35 ± 0.35, P = 0.002). Protein levels confirmed the same expression pattern. MMP-13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability. Conclusion: These results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 (MMP-13), a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs). The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis. Bone marrow-derived dendritic cells were obtained from C57BL/6 mice. One short-interfering RNA specific for MMP-13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry. Compared to the control DCs, MMP-13-silenced DCs increased expression of anti-apoptosis-related genes, BAG1 (control group vs. MMP-13-silenced group: 4.08 ± 0.60 vs. 6.11 ± 0.87, P = 0.008), BCL-2 (control group vs. MMP-13-silenced group: 7.54 ± 0.76 vs. 9.54 ± 1.29, P = 0.036), and TP73 (control group vs. MMP-13-silenced group: 4.33 ± 0.29 vs. 5.60 ± 0.32, P = 0.001) and decreased apoptosis-related genes, CASP1 (control group vs. MMP-13-silenced group: 3.79 ± 0.67 vs. 2.54 ± 0.39, P = 0.019), LTBR (control group vs. MMP-13-silenced group: 9.23 ± 1.25 vs. 6.24 ± 1.15, P = 0.012), and CASP4 (control group vs. MMP-13-silenced group: 2.07 ± 0.56 vs. 0.35 ± 0.35, P = 0.002). Protein levels confirmed the same expression pattern. MMP-13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability. These results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
Background: Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 (MMP-13), a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs). The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis. Methods: Bone marrow-derived dendritic cells were obtained from C57BL/6 mice. One short-interfering RNA specific for MMP-13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry. Results: Compared to the control DCs, MMP-13-silenced DCs increased expression of anti-apoptosis-related genes, BAG1 (control group vs. MMP-13-silenced group: 4.08 +- 0.60 vs. 6.11 +- 0.87, P = 0.008), BCL-2 (control group vs. MMP-13-silenced group: 7.54 +- 0.76 vs. 9.54 +- 1.29, P = 0.036), and TP73 (control group vs. MMP-13-silenced group: 4.33 +- 0.29 vs. 5.60 +- 0.32, P = 0.001) and decreased apoptosis-related genes, CASP1 (control group vs. MMP-13-silenced group: 3.79 +- 0.67 vs. 2.54 +- 0.39, P = 0.019), LTBR (control group vs. MMP-13-silenced group: 9.23 +- 1.25 vs. 6.24 +- 1.15, P = 0.012), and CASP4 (control group vs. MMP-13-silenced group: 2.07 +- 0.56 vs. 0.35 +- 0.35, P = 0.002). Protein levels confirmed the same expression pattern. MMP-13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability. Conclusion: These results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
Audience Academic
Author Xiao-Dong Li Xin-Rui Zhang Zhi-Hao Li Yang Yang Duo Zhang Heng Zheng Shu-Ying Dong Juan Chen Xian-Dong Zeng
AuthorAffiliation Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China Department of Dermatology Key Laboratory of Immunodermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning 110001, China Department of Oncology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China
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– name: 2 Department of Dermatology Key Laboratory of Immunodermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning 110001, China
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/28303856$$D View this record in MEDLINE/PubMed
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CitedBy_id crossref_primary_10_3390_pathogens10050527
crossref_primary_10_1016_j_aquaculture_2018_08_033
crossref_primary_10_1186_s12935_023_03116_0
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ContentType Journal Article
Copyright COPYRIGHT 2017 Medknow Publications and Media Pvt. Ltd.
Copyright Medknow Publications & Media Pvt. Ltd. Mar 20, 2017
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DocumentTitleAlternate Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells
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Issue 6
Keywords Matrix Metallopeptidase 13
Maturation
Phagocytosis
Dendritic Cell
Apoptosis
Language English
License This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
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Notes Background： Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 （MMP- 13）, a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells （DCs）. The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis. Methods： Bone marrow-derived dendritic cells were obtained fiom C57BL/6 mice. One short-interfering RNA specific for MMP- 13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry. Results： Compared to the control DCs, MMP- 13-silenced DCs increased expression of anti-apoptosis-related genes, BAGl （control group vs. MMP-13-silenced group： 4.08 ± 0.60 vs. 6.11 ± 0.87, P = 0.008）, BCL-2 （control group vs. MMP-13-silenced group： 7.54 ± 0.76 vs. 9.54 ± 1.29, P = 0.036）, and TP73 （control group vs. MMP- 13-silenced group： 4.33 ± 0.29 vs. 5.60 ±0.32, P = 0.001 ） and decreased apoptosis-related genes, CASPI （control group vs. MMP- 13-silenced group： 3.79±0.67 vs. 2.54±0.39, P - 0.019）, LTBR （control group vs. MMP- 13-silenced group： 9.23 ±1.25 vs. 6.24 ± 1.15, P = 0.012）, and CASP4 （control group vs. MMP-13-silenced group： 2.07 ± 0.56 vs. 0.35 ±0.35, P = 0.002）. Protein levels confirmed the same expression pattern. MMP- 13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability. Conclusion： These results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
Apoptosis; Dendritic Cell; Matrix Metallopeptidase 13： Maturation： Phagocytosis
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OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358423/
PMID 28303856
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PublicationDate 2017-03-20
PublicationDateYYYYMMDD 2017-03-20
PublicationDate_xml – month: 03
  year: 2017
  text: 2017-03-20
  day: 20
PublicationDecade 2010
PublicationPlace China
PublicationPlace_xml – name: China
– name: Baltimore
– name: India
PublicationTitle Chinese medical journal
PublicationTitleAlternate Chinese Medical Journal
PublicationTitle_FL Chinese Medical Journal
PublicationYear 2017
Publisher Medknow Publications and Media Pvt. Ltd
Lippincott Williams & Wilkins Ovid Technologies
Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China%Department of Dermatology Key Laboratory of Immunodermatology, No.1 Hospital of China Medical University, Shenyang, Liaoning 110001, China%Department of Oncology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China
Medknow Publications & Media Pvt Ltd
Wolters Kluwer
Publisher_xml – name: Medknow Publications and Media Pvt. Ltd
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– name: Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China%Department of Dermatology Key Laboratory of Immunodermatology, No.1 Hospital of China Medical University, Shenyang, Liaoning 110001, China%Department of Oncology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China
– name: Medknow Publications & Media Pvt Ltd
– name: Wolters Kluwer
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Snippet Background： Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture...
Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as...
Background: Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture...
BACKGROUNDDendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens...
Background:Dendritic cells are professional antigen-presenting ceils found in an immature state in epithelia and interstitial space,where they capture antigens...
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SubjectTerms Animals
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Apoptosis; Dendritic Cell; Matrix Metallopeptidase 13; Maturation; Phagocytosis
Bone marrow
Bone Marrow Cells - cytology
DCS控制系统
Dendritic cells
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - metabolism
Dermatology
Experiments
Female
Flow cytometry
Gene expression
Health aspects
Hospitals
Immunoglobulins
Inflammation
Lipopolysaccharides - pharmacology
Matrix Metalloproteinase 13 - metabolism
Matrix Metalloproteinase 13 - physiology
Matrix metalloproteinase inhibitors
Medical research
Mice
Mice, Inbred C57BL
Original
Polymerase chain reaction
Proteins
RNA, Small Interfering
Western印迹法
凋亡相关基因
基质金属蛋白酶
小鼠骨髓
抗原呈递细胞
树突状细胞
细胞功能
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Title Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells
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