Cerebral Organoids Recapitulate Epigenomic Signatures of the Human Fetal Brain

• Published in: Cell reports (Cambridge) Vol. 17; no. 12; pp. 3369 - 3384
• Main Authors: Luo, Chongyuan, Lancaster, Madeline A., Castanon, Rosa, Nery, Joseph R., Knoblich, Juergen A., Ecker, Joseph R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.12.2016; Elsevier
• Subjects: 3D culture; Brain - growth & development; Brain - metabolism; brain development; Cell Differentiation - genetics; DNA methylation; DNA Methylation - genetics; epigenome; Epigenomics; Gene Expression Regulation, Developmental; Humans; organoid; Organoids - growth & development; Organoids - metabolism; Pluripotent Stem Cells - metabolism; Tissue Culture Techniques; Transcriptome - genetics; brain development; DNA methylation; epigenome; 3D culture; organoid

Organoids derived from human pluripotent stem cells recapitulate the early three-dimensional organization of the human brain, but whether they establish the epigenomic and transcriptional programs essential for brain development is unknown. We compared epigenomic and regulatory features in cerebral...