In vivo biodistribution analysis of transmission competent and defective RNA virus-based episomal vector

• Published in: Scientific reports Vol. 10; no. 1; pp. 5890 - 12
• Main Authors: Komatsu, Yumiko, Tanaka, Chiaki, Komorizono, Ryo, Tomonaga, Keizo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.04.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/2300; 631/326/596/2561; Animals; Animals, Genetically Modified; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; Astrocytes; Astrocytes - metabolism; Body weight; Brain - metabolism; CD8 antigen; Feasibility Studies; Feces - virology; Female; Gene Expression; Gene transfer; Genetic Vectors - administration & dosage; Genetic Vectors - genetics; Genetic Vectors - isolation & purification; Genetic Vectors - pharmacokinetics; Humanities and Social Sciences; Lymphocytes T; Microglia; Microglia - metabolism; multidisciplinary; Neurons - metabolism; Pathogenicity; Pathogens; Plasmids - genetics; Rats; Rats, Inbred Lew; RNA viruses; RNA Viruses - genetics; RNA Viruses - immunology; RNA Viruses - isolation & purification; Science; Science (multidisciplinary); Tissue Distribution; Transduction, Genetic; Transgenes - genetics; Tropism; Virus Replication; Virus Shedding

RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro . However, little is known about its tissue tropism and persistence of gene expression in vivo . Here, to evaluate the feasibility of REVec for...