Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission

• Published in: Science (American Association for the Advancement of Science) Vol. 286; no. 5443; pp. 1347 - 1350
• Main Authors: Jonas, Elizabeth A., Buchanan, JoAnn, Kaczmarek, Leonard K.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 12.11.1999; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Action Potentials; Animals; Aquatic life; Biochemistry. Physiology. Immunology; Biological and medical sciences; Calcium; Calcium - metabolism; Calcium Channels - metabolism; Cell membranes; Cell organelles; Cellular biology; Decapodiformes; Electric Conductivity; Electric Stimulation; Electrodes; Fundamental and applied biological sciences. Psychology; Intracellular Membranes - metabolism; Invertebrates; Ion channels; Ion Channels - metabolism; Ion Transport; Ions; Membranes; Microscopy, Electron; Mitochondria; Mitochondria - metabolism; Mollusca; Organelles; P branes; Patch-Clamp Techniques; Physiology. Development; Pipettes; Porins - metabolism; Presynaptic Terminals - metabolism; Presynaptic Terminals - ultrastructure; Synapses; Synaptic Transmission; Time Factors; Voltage-Dependent Anion Channels; Mitochondria; Synaptic transmission; Electrophysiology; Patch clamp method; Ionic channel; Ionic conductance; Invertebrata; Mollusca

Although ion channels have been detected in mitochondria, scientists have not been able to record ion transport in mitochondria of intact cells. A variation of the patch clamp technique was used to record ion channel activity from intracellular organelles in the presynaptic terminal of the squid. El...