Tracking the roots of cellulase hyperproduction by the fungus Trichoderma reesei using massively parallel DNA sequencing

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 38; pp. 16151 - 16156
• Main Authors: Le Crom, Stéphane, Schackwitz, Wendy, Pennacchio, Len, Magnuson, Jon K, Culley, David E, Collett, James R, Martin, Joel, Druzhinina, Irina S, Mathis, Hugues, Monot, Frédéric, Seiboth, Bernhard, Cherry, Barbara, Rey, Michael, Berka, Randy, Kubicek, Christian P, Baker, Scott E, Margeot, Antoine
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 22.09.2009; National Acad Sciences
• Subjects: 09 BIOMASS FUELS; Base Composition; Biochemistry, Molecular Biology; Biodiesel fuels; BIOFUELS; Biological Sciences; BIOMASS; biosynthesis; CARBON; CELLULASE; Cellulase - genetics; Cellulase - metabolism; cellulase hyperproducing strains; cellulases; Chemicals; Clear cutting; Deoxyribonucleic acid; DNA; DNA SEQUENCING; DNA, Fungal; DNA, Fungal - chemistry; DNA, Fungal - genetics; ETHANOL; Fuel technology; FUNCTIONALS; Fungal Proteins; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Gene deletion; GENES; Genes, Fungal; Genetic mutation; genetic techniques and protocols; genome; Genome, Fungal; Genome, Fungal - genetics; Genomes; Genomics; Genotype & phenotype; HYDROLYSIS; Hypocrea jecorina; Industrial applications; Life Sciences; Messenger RNA; METABOLISM; mRNA stability; MUTAGENESIS; MUTANTS; Mutation; MUTATIONS; Nuclear transport; nucleotide sequences; NUCLEOTIDES; parallel DNA sequencing; Perfect state; PHENOTYPE; Polymorphism, Single Nucleotide; Quantitative Methods; Roots; SACCHARIDES; Secretion; sequence analysis; Sequence Analysis, DNA; Sequence Analysis, DNA - methods; Sequencing; Species Specificity; STRAINS; Sugar; TRANSCRIPTION; Trichoderma; Trichoderma - classification; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma reesei; TRICHODERMA VIRIDE; Trichoderma, cellulase, fungi, genomics

Trichoderma reesei (teleomorph Hypocrea jecorina) is the main industrial source of cellulases and hemicellulases harnessed for the hydrolysis of biomass to simple sugars, which can then be converted to biofuels such as ethanol and other chemicals. The highly productive strains in use today were gene...