DNA fragmentation of lymphocytes and sperm cells induced by nickel released from orthodontic archwires: A preliminary study

Orthodontic brackets and archwires placed intraorally are subject to corrosion, leading to the release of cytotoxic metal ions. The aim of this study was to determine whether the use of orthodontic NiTi archwires increases systemic Ni levels and cause alterations on the DNA of cells unrelated to the...

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Bibliographic Details
Published inDental Materials Journal Vol. 43; no. 4; pp. 539 - 545
Main Authors UMAÑA, José Alfredo FACIO, Roberto BELTRAN DEL RIO PARRA, MARTÍNEZ, Raúl Uriel MEDINA, MARTÍNEZ, Nadia BETANCOURT, LUNA, Pedro IV GONZÁLEZ, MARTÍNEZ, Javier MORÁN
Format Journal Article
LanguageEnglish
Published Japan The Japanese Society for Dental Materials and Devices 25.07.2024
Japanese Society for Dental Materials and Devices
Japan Science and Technology Agency
Subjects
Adult
Archwires
Bioassays
Comet Assay
Concentration cell corrosion
Corrosion cell
Corrosion tests
Cytotoxicity
Damage detection
Deoxyribonucleic acid
DNA
DNA damage
DNA Fragmentation
Fragmentation
Humans
Lymphocytes
Lymphocytes - drug effects
Male
Metal ions
Nickel
Nickel compounds
NiTi archwires
Orthodontic Wires
Orthodontics
Placement
Semen
Sperm
Sperm cells
Spermatozoa - drug effects
Structural damage
Titanium
Urine
NiTi archwires
Lymphocytes
Sperm cells
DNA fragmentation
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Summary:Orthodontic brackets and archwires placed intraorally are subject to corrosion, leading to the release of cytotoxic metal ions. The aim of this study was to determine whether the use of orthodontic NiTi archwires increases systemic Ni levels and cause alterations on the DNA of cells unrelated to the oral environment such as lymphocytes and sperm cells. Human urine, semen and blood samples were collected before (baseline) sham placement of orthodontic archwires and 15 and 30 days after placement. Lymphocytes and sperm cells cells were evaluated by comet assay. Ni concentration levels in urine increased significantly between baseline and 15 days (p<0.01) and 15 and 30 days of exposure (p<0.01). Progressive decrease in sperm viability and motility was observed between the sampling periods. Lymphocytes and sperm cells showed DNA fragmentation. The increase in systemic concentration of nickel induced structural damage in the DNA of lymphocytes and human sperm cells.
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ISSN:0287-4547
1881-1361
1881-1361
DOI:10.4012/dmj.2023-286