Synthetic production of prenylated naringenins in yeast using promiscuous microbial prenyltransferases

• Published in: Metabolic Engineering Communications Vol. 12; p. e00169
• Main Authors: Isogai, Shota, Okahashi, Nobuyuki, Asama, Ririka, Nakamura, Tomomi, Hasunuma, Tomohisa, Matsuda, Fumio, Ishii, Jun, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2021; Elsevier BV; Elsevier
• Subjects: biochemical pathways; Biology (General); Biotechnology; dimethylallyltranstransferase; Full Length; genomics; Naringenin; phenylalanine; Prenylflavonoids; Prenylnaringenin; Prenyltransferase; QH301-705.5; regioselectivity; synthesis; TP248.13-248.65; tryptophan; Yeast; yeasts; Prenylflavonoids; Prenyltransferase; Naringenin; Yeast; Prenylnaringenin

Reconstitution of prenylflavonoids using the flavonoid biosynthetic pathway and prenyltransferases (PTs) in microbes can be a promising attractive alternative to plant-based production or chemical synthesis. Here, we demonstrate that promiscuous microbial PTs can be a substitute for regiospecific bu...