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Keywords: 1678/631/45/269; 1877/631/532/2437; 347/631/1647/1407/652; 600/631/1647/767/2199; Animals; Biological Assay - methods; Biomedicine; Biotechnology; Cancer Research; Cells, Cultured; Cystic fibrosis; Cystic Fibrosis - diagnosis; Cystic Fibrosis - genetics; Cystic Fibrosis - pathology; Cystic Fibrosis Transmembrane Conductance Regulator - analysis; Cystic Fibrosis Transmembrane Conductance Regulator - genetics; Cystic Fibrosis Transmembrane Conductance Regulator - metabolism; Drug therapy; Female; HEK293 Cells; Humans; Incubation; Infectious Diseases; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Low temperature; Male; Metabolic Diseases; Mice; Mice, Inbred C57BL; Mice, Inbred CFTR; Molecular Medicine; Mutagenesis; Mutant Proteins - analysis; Mutant Proteins - genetics; Mutant Proteins - metabolism; Mutant Proteins - physiology; Mutation; Neurosciences; Organoids - metabolism; Organoids - pathology; Primary Cell Culture - methods; technical-report; Technology application; Tissues; Water - metabolism; Netherlands