Pattern extraction of structural responses of gut microbiota to rotavirus infection via multivariate statistical analysis of clone library data

• Published in: FEMS microbiology ecology Vol. 70; no. 2; pp. 177 - 185
• Main Authors: Zhang, Meiling, Zhang, Menghui, Zhang, Chenhong, Du, Huimin, Wei, Guifang, Pang, Xiaoyan, Zhou, Haokui, Liu, Binbin, Zhao, Liping
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.11.2009; Blackwell Publishing Ltd; Oxford University Press
• Subjects: Bacteroides; Bacteroides - classification; Bacteroides - genetics; Bacteroides - growth & development; Bacteroides fragilis; Bacteroides stercoris; Bacteroides vulgatus; Biodiversity; Child, Preschool; children; classification; clone library; clones; Cloning; Discriminant analysis; DNA, Bacterial; DNA, Bacterial - genetics; Ecology; feces; Feces - microbiology; Female; Gastrointestinal Tract; Gastrointestinal Tract - microbiology; Gene Library; Gene sequencing; genetics; growth & development; Humans; Infant; Intestinal microflora; intestinal microorganisms; Least-Squares Analysis; Libraries; Male; Microbiology; Microbiota; Multivariate Analysis; Multivariate statistical analysis; partial least squares; Principal Component Analysis; ribosomal RNA; RNA, Ribosomal, 16S; RNA, Ribosomal, 16S - genetics; Rotavirus; Rotavirus Infections; Rotavirus Infections - microbiology; rRNA 16S; Sequence Analysis, DNA; Statistical analysis; Statistical methods; Statistics; Viruses; partial least squares; rotavirus; clone library

This study developed a new statistical strategy for analyzing clone library data to observe whether there is a defined pattern in structural responses of gut microbiota to environmental perturbations. A large clone library of genus Bacteroides was constructed with fecal samples for each subject in r...