The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay

• Published in: The EMBO journal Vol. 20; no. 17; pp. 4987 - 4997
• Main Authors: Le Hir, Hervé, Gatfield, David, Izaurralde, Elisa, Moore, Melissa J.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 03.09.2001; Nature Publishing Group UK; Springer Nature B.V; Oxford University Press
• Subjects: Animals; Antigens, Nuclear; Binding Sites; Cloning, Molecular; Decay; DEK protein; DNA-Binding Proteins - metabolism; Escherichia coli; exon-exon junction complex; Exons; Humans; In Vitro Techniques; Models, Genetic; mRNA decay; mRNA export; nonsense-mediated decay; Nuclear Matrix-Associated Proteins; Nuclear Proteins - metabolism; Oocytes - physiology; pre-mRNA splicing; Recombinant Proteins - metabolism; REF protein; Ribonucleoproteins; RNA Precursors - metabolism; RNA Splicing; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA-Binding Proteins - metabolism; RNPS1 protein; SRm160 protein; Xenopus; Xenopus laevis; Y14 protein; pre‐mRNA splicing; mRNA export; nonsense‐mediated decay; exon–exon junction complex

We recently reported that spliceosomes alter messenger ribonucleoprotein particle (mRNP) composition by depositing several proteins 20–24 nucleotides upstream of mRNA exon–exon junctions. When assembled in vitro , this so‐called ‘exon–exon junction complex’ (EJC) contains at least five proteins: SRm...