The perils of PCR: can we accurately ‘correct’ antimalarial trials?
During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates. The epidemiological, biological and technical limitations of PCR correction and...
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| Published in | Trends in parasitology Vol. 26; no. 3; pp. 119 - 124 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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Amsterdam
Elsevier Ltd
01.03.2010
Elsevier |
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| Abstract | During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates. The epidemiological, biological and technical limitations of PCR correction and how this can lead to misclassification in drug trial outcomes are underappreciated. This article considers these limitations and proposes a framework for reporting, interpreting and improving PCR correction of antimalarial trials. |
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| AbstractList | During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates. The epidemiological, biological and technical limitations of PCR correction and how this can lead to misclassification in drug trial outcomes are underappreciated. This article considers these limitations and proposes a framework for reporting, interpreting and improving PCR correction of antimalarial trials. During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates. The epidemiological, biological and technical limitations of PCR correction and how this can lead to misclassification in drug trial outcomes are underappreciated. This article considers these limitations and proposes a framework for reporting, interpreting and improving PCR correction of antimalarial trials.During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates. The epidemiological, biological and technical limitations of PCR correction and how this can lead to misclassification in drug trial outcomes are underappreciated. This article considers these limitations and proposes a framework for reporting, interpreting and improving PCR correction of antimalarial trials. During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates. The epidemiological, biological and technical limitations of PCR correction and how this may lead to misclassification in drug trial outcomes are underappreciated. This article considers these limitations and proposes a framework for reporting, interpreting, and improving PCR correction of antimalarial trials. |
| Author | Gadalla, Nahla Meshnick, Steven R. Juliano, Jonathan J. Sutherland, Colin J. |
| AuthorAffiliation | 1 Division of Infectious Diseases, Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC, USA 2 Dept of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK 3 Dept of Clinical Parasitology, Hospital for Tropical Diseases, London, UK 4 Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, NC, USA |
| AuthorAffiliation_xml | – name: 1 Division of Infectious Diseases, Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC, USA – name: 2 Dept of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK – name: 3 Dept of Clinical Parasitology, Hospital for Tropical Diseases, London, UK – name: 4 Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, NC, USA |
| Author_xml | – sequence: 1 givenname: Jonathan J. surname: Juliano fullname: Juliano, Jonathan J. organization: Division of Infectious Diseases, Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA – sequence: 2 givenname: Nahla surname: Gadalla fullname: Gadalla, Nahla organization: Department of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK, WC1E 7HT – sequence: 3 givenname: Colin J. surname: Sutherland fullname: Sutherland, Colin J. organization: Department of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK, WC1E 7HT – sequence: 4 givenname: Steven R. surname: Meshnick fullname: Meshnick, Steven R. email: meshnick@email.unc.edu organization: Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, NC 27599, USA |
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| SubjectTerms | Animal aquaculture Animal productions Antimalarials - therapeutic use Biological and medical sciences Clinical Trials as Topic - methods Crustacea Drugs Fundamental and applied biological sciences. Psychology Gastroenterology and Hepatology Humans Infectious Disease Invertebrate aquaculture Malaria - drug therapy Polymerase Chain Reaction - methods |
| Title | The perils of PCR: can we accurately ‘correct’ antimalarial trials? |
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