Exploitation of gene(s) involved in 2,4-diacetylphloroglucinol biosynthesis to confer a new biocontrol capability to a Pseudomonas strain

• Published in: Applied and Environmental Microbiology Vol. 58; no. 12; pp. 3873 - 3878
• Main Authors: Fenton, A.M. (University College, Cork, Ireland), Stephens, P.M, Crowley, J, O'Callaghan, M, O'Gara, F
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.1992
• Subjects: analogs & derivatives; Anti-Bacterial Agents; Anti-Bacterial Agents - metabolism; ANTIBIOTICOS; antibiotics; ANTIBIOTIQUE; BETA VULGARIS; Biological and medical sciences; Biological control; Biology of microorganisms of confirmed or potential industrial interest; BIOSINTESIS; BIOSYNTHESE; Biosynthesis; Biotechnology; CONTROL BIOLOGICO; CONTROL DE ENFERMEDADES; CONTROLE DE MALADIES; disease control; Fundamental and applied biological sciences. Psychology; GENE; Gene Expression; GENES; Genes, Bacterial; Genetics; GRAINE; LUTTE BIOLOGIQUE; metabolism; microbiology; Mission oriented research; Phloroglucinol; Phloroglucinol - analogs & derivatives; Phloroglucinol - metabolism; plant diseases and disorders; Plants; Plants - microbiology; PSEUDOMONAS; Pseudomonas - genetics; Pseudomonas - metabolism; PYTHIUM ULTIMUM; seeds; SEMILLA; Sugar; Vegetables; Pseudomonadales; Genetic transformation; Phycomycetes; Biosynthesis; Pseudomonas; Fungi; Rhizosphere; Antagonism; Pythium ultimum; Gene; Mutagenesis; Bacteria; Pseudomonadaceae; Sugar beet; Complementation; Colonization; Thallophyta

Tn5 mutagenesis and complementation analysis were used to clone a 6-kb genomic fragment required for biosynthesis of 2,4-diacetylphloroglucinol (Phl) from fluorescent Pseudomonas sp. strain F113. A recombinant plasmid, pCU203, containing this region partially complemented a Phl production-negative m...