Rapid profiling of drug-resistant bacteria using DNA-binding dyes and a nanopore-based DNA sequencer

• Published in: Scientific reports Vol. 11; no. 1; p. 3436
• Main Authors: Ohno, Ayumu, Umezawa, Kazuo, Asai, Satomi, Kryukov, Kirill, Nakagawa, So, Miyachi, Hayato, Imanishi, Tadashi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.02.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/2217; 631/1647/2234; 631/1647/514; 631/208/212; 631/208/2489; 631/208/325; 692/4017; Antibiotics; Bacteria; Deoxyribonucleic acid; DNA; Drug resistance; Humanities and Social Sciences; multidisciplinary; Nucleotide sequence; rRNA 16S; Science; Science (multidisciplinary)

Spread of drug-resistant bacteria is a serious problem worldwide. We thus designed a new sequence-based protocol that can quickly identify bacterial compositions of clinical samples and their drug-resistance profiles simultaneously. Here we utilized propidium monoazide (PMA) that prohibits DNA ampli...