Determination of Anthraquinone-Tagged Amines Using High-Performance Liquid Chromatography with Online UV Irradiation and Luminol Chemiluminescence Detection

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 5; p. 2146
• Main Authors: Kishikawa, Naoya, El-Maghrabey, Mahmoud, Kawamoto, Ayaka, Ohyama, Kaname, Kuroda, Naotaka
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.02.2023; MDPI
• Subjects: amine; Amines; Analysis; Anthraquinone; Anthraquinones; Biogenic amines; Biogenic Amines - analysis; Carbonyl compounds; Carbonyls; Chemiluminescence; Chloride; Chlorides; Chromatography; Chromatography, High Pressure Liquid - methods; derivatization; High performance liquid chromatography; Irradiation; Liquid chromatography; Luminescence; Luminol; Luminol - chemistry; Methods; Phenethylamine; Phenethylamines; Phosgene; Quinone; Quinones; Quinones - analysis; Reactive Oxygen Species - chemistry; Reagents; Tryptamine; Tryptamines; Ultraviolet radiation; UV irradiation; Japan; anthraquinone; UV irradiation; derivatization; chemiluminescence; amine

Quinones are frequently used as derivatization reagents in HPLC analysis to improve detection sensitivity. In the present study, a simple, sensitive, and selective chemiluminescence (CL) derivatization strategy for biogenic amines, prior to their HPLC-CL analysis, was developed. The novel CL derivat...