Optimal Detection of Serum Antipaternal Antileukocytic Antibodies after Injection of Allogenic Lymphocytes in Women with Habitual Abortions

• Published in: Bulletin of experimental biology and medicine Vol. 153; no. 5; pp. 726 - 729
• Main Authors: Krechetova, L. V., Nikolaeva, M. A., Van’ko, L. V., Ziganshina, M. M., Golubeva, E. L., Stepanova, E. O., Sukhikh, G. T.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.09.2012; Springer; Springer Nature B.V
• Subjects: Abortion; Abortion, Habitual - blood; Abortion, Habitual - prevention & control; Antibodies; Antilymphocyte Serum - isolation & purification; Biomedical and Life Sciences; Biomedicine; Cell Biology; Female; Flow Cytometry - methods; Humans; Immunization - methods; Internal Medicine; Laboratory Medicine; Lymphocyte Transfusion - methods; Lymphocytes; Male; Monocytes - immunology; Pathology; Pregnancy; Transplantation, Homologous - methods; Viral antibodies; Women; antipaternal antileukocytic antibodies; flow cytometry; alloimmunization
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-012-1811-8

The level of antipaternal antileukocytic antibodies detected by flow cytometry is a parameter reflecting the efficiency of alloimmunization of women with reproductive disorders during preparation to pregnancy. The results of evaluation of antipaternal antileukocytic antibodies by two modifications of the method are presented. The optimal method for detection of antipaternal antileukocytic antibodies after immunocytotherapy is selected.