Methods for the directed evolution of proteins

• Published in: Nature reviews. Genetics Vol. 16; no. 7; pp. 379 - 394
• Main Authors: Packer, Michael S., Liu, David R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.07.2015; Nature Publishing Group
• Subjects: 49/40; 631/1647/1513; 631/1647/2163; 631/181/735; 631/208/191/1908; 631/61/338; Agriculture; Animal Genetics and Genomics; Animals; Biomedicine; Cancer Research; Cellular proteins; Directed Molecular Evolution; Gene Function; Genetic regulation; Genetic research; Genetic Techniques; Human Genetics; Humans; Properties; Protein Engineering - methods; Proteins - genetics; Proteins - metabolism; review-article; Substrate Specificity

Key Points Directed evolution is a cyclic process that alternates between gene diversification and screening for or selection of functional gene variants. Library size limitations can be overcome by focusing library diversity on residues implicated by molecular structures, computational models or ph...