在水稻纹枯病菌菌核形成中5个基因的表达差异分析

以水稻纹枯病菌（Rhizoctonia solani AG-1 IA） 菌株GDll8不同生长时期的总RNA为材料，以18SrRNA作为内参基因，根据实验室抑制消减杂交（suppression subtractive hybridization, SSH） 已获得的立枯丝核菌基因片段的表达序列标签（expressed sequence tags, ESTs）设计引物，采用实时定量RT-PCR检测A、B、C、E、F基因（分别为双组分反应调节子、激活巨噬细胞糖蛋白、胞外金属弹力蛋白酶、亲环蛋白、内质网囊泡蛋白ERV29）的表达，以期找到水稻纹枯病菌菌核形成中的差异表达基因结果表明：从菌核开始形成到...

Full description

Saved in:
Bibliographic Details
Published in浙江大学学报（农业与生命科学版） Vol. 39; no. 1; pp. 18 - 25
Main Author 王伟 杨惠 王政逸 陈卫良
Format Journal Article
LanguageChinese
Published 浙江大学生物技术研究所水稻生物学国家重点实验室,杭州,310058 2013
Subjects
PCR
基因差异表达
实时荧光定量RT
水稻纹枯病菌
菌核形成
基因差异表达
水稻纹枯病菌
菌核形成
实时荧光定量RT-PCR
Online AccessGet full text

Cover

More Information
Summary:以水稻纹枯病菌（Rhizoctonia solani AG-1 IA） 菌株GDll8不同生长时期的总RNA为材料，以18SrRNA作为内参基因，根据实验室抑制消减杂交（suppression subtractive hybridization, SSH） 已获得的立枯丝核菌基因片段的表达序列标签（expressed sequence tags, ESTs）设计引物，采用实时定量RT-PCR检测A、B、C、E、F基因（分别为双组分反应调节子、激活巨噬细胞糖蛋白、胞外金属弹力蛋白酶、亲环蛋白、内质网囊泡蛋白ERV29）的表达，以期找到水稻纹枯病菌菌核形成中的差异表达基因结果表明：从菌核开始形成到菌核成熟的过程中，这5个基因的表达量逐渐降低，且菌核在成熟时表达量最低，但成熟后这些基因的表达量迅速恢复到之前的水平，之后表达量基本保持不变．提示这5个基因在水稻纹枯病菌菌核形成的不同时期的表达有显著差异，说明这5个基因可能受菌核形成过程中胁迫条件的诱导表达．
Bibliography:Summary Rhizoctonia solani is an important plant distribution, and the sclerotia are the main source of pathogenic fungus with a wide host range and worldwide infection for the diseases caused by this pathogen. The mechanism of formation and development of sclerotia of Rhizoctonia solan had been investigated by some researches, but they were mainly focused on the relationship between the sclerotium formation and the nutrition or environmental factors. There is still lack of the knowledge on the molecular basis of sclerotium formation of R. solani, and little is known about the sclerotium formation-related genes. Subtractive cDNA library of differentially expressed genes during the sclerotium formation of R. solani AG 1 IA （GI〉118 strain） was constructed previously by using suppression subtractive hybridization （SSH） technique in our laboratory. The hyphal and sclerotium mRNA was used as the driver and tester, respectively. In the screening, some specific sclerotium formation related gene fragments were identi
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2011.11.221