Proximity labeling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID

• Published in: eLife Vol. 8
• Main Authors: Mair, Andrea, Xu, Shou-Ling, Branon, Tess C, Ting, Alice Y, Bergmann, Dominique C
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 19.09.2019; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: Animal behavior; Arabidopsis; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biotin; biotin ligase; Cells (Biology); DNA; Enzymes; FAMA; Genes; guard cell; Housing; Ligases; Molecular biology; Multiprotein Complexes - metabolism; N. benthamiana; Novels; nuclear proteome; Organelles - metabolism; Physiological aspects; Plant Biology; Plant physiology; Plant proteins; Plants (Organisms); Protein interaction; Protein purification; Proteins; Proteome - metabolism; Proteomes; proximity labeling; Staining and Labeling - methods; Stomata; Tools and Resources; Transcription factors; United States; proximity labeling; nuclear proteome; A. thaliana; plant biology; biotin ligase; guard cell; N. benthamiana; FAMA
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.47864

Defining specific protein interactions and spatially or temporally restricted local proteomes improves our understanding of all cellular processes, but obtaining such data is challenging, especially for rare proteins, cell types, or events. Proximity labeling enables discovery of protein neighborhoo...