An analysis of differential gene expression in peripheral nerve and muscle utilizing RNA sequencing after polyethylene glycol nerve fusion in a rat sciatic nerve injury model

• Published in: PloS one Vol. 19; no. 9; p. e0304773
• Main Authors: Weiss, Samantha N., Legato, Joseph M., Liu, Yichuan, Vaccaro, Courtney N., Da Silva, Renata Pellegrino, Miskiel, Sandra, Gilbert, Grace V., Hakonarson, Hakon, Fuller, David A., Buono, Russell J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.09.2024; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Bioinformatics; Biology and Life Sciences; Care and treatment; Clinical outcomes; Composition; Degeneration; Diagnosis; Disease Models, Animal; Gene expression; Gene Expression Profiling; Gene Expression Regulation - drug effects; Gene fusion; Gene regulation; Gene sequencing; Genetic aspects; Injuries; Injury analysis; Injury prevention; Innervation; Laboratory animals; Male; Medical examination; Medicine and Health Sciences; Molecular modelling; Muscle, Skeletal - drug effects; Muscle, Skeletal - innervation; Muscle, Skeletal - metabolism; Muscles; Nerve Regeneration - drug effects; Nerve Regeneration - genetics; Nervous system; Neurodegeneration; Patient satisfaction; Peripheral nerve diseases; Peripheral Nerve Injuries - genetics; Peripheral nerves; Polyethylene glycol; Polyethylene Glycols - pharmacology; Rats; Rats, Inbred Lew; Recovery; Recovery of function; Research and analysis methods; Ribonucleic acid; RNA; RNA sequencing; Sciatic nerve; Sciatic Nerve - injuries; Sequence Analysis, RNA; Sutures; Trauma; United States

Application of polyethylene glycol (PEG) to a peripheral nerve injury at the time of primary neurorrhaphy is thought to prevent Wallerian degeneration via direct axolemma fusion. The molecular mechanisms of nerve fusion and recovery are unclear. Our study tested the hypothesis that PEG alters gene e...