Buccal cells DNA extraction to obtain high quality human genomic DNA suitable for polymorphism genotyping by PCR-RFLP and Real-Time PCR

• Published in: Journal of applied oral science Vol. 20; no. 4; pp. 467 - 471
• Main Authors: Küchler, Erika Calvano, Tannure, Patricia Nivoloni, Falagan-Lotsch, Priscila, Lopes, Taliria Silva, Granjeiro, Jose Mauro, Amorim, Lidia Maria Fonte
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Faculdade de Odontologia de Bauru da Universidade de São Paulo 01.08.2012; Faculdade De Odontologia De Bauru - USP; University of São Paulo
• Subjects: Adult; Analysis of Variance; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; DNA; DNA - isolation & purification; Electrophoresis; Female; Genetic polymorphism; Genotyping Techniques - methods; Humans; Male; Middle Aged; Mouth - cytology; Original; PCR; Polymorphism, Restriction Fragment Length - genetics; Real-Time Polymerase Chain Reaction - methods; Reproducibility of Results; Saliva; Spectrophotometry; Time Factors; Saliva; DNA; PCR; Genetic polymorphism

The aim of this study was to evaluate, by PCR-RFLP and real-time PCR, the yield and quality of genomic DNA collected from buccal cells by mouthwash after different storage times at room temperature. A group of volunteers was recruited to collect buccal cells using a mouthwash solution. The collected...