Development and evaluation of yeast-based GFP and luciferase reporter assays for chemical-induced genotoxicity and oxidative damage

• Published in: Applied microbiology and biotechnology Vol. 101; no. 2; pp. 659 - 671
• Main Authors: Suzuki, Hajime, Sakabe, Takahiro, Hirose, Yuu, Eki, Toshihiko
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2017; Springer; Springer Nature B.V
• Subjects: Analysis; Applied Genetics and Molecular Biotechnology; Bioassay; Bioassays; Biomedical and Life Sciences; Biotechnology; Cell cycle; Chemicals; Deoxyribonucleic acid; DNA; DNA damage; DNA repair; Fluorescence; Genes; Genes, Reporter; Genetic engineering; Genomes; Genotoxicity; green fluorescent protein; Green Fluorescent Proteins - analysis; Green Fluorescent Proteins - genetics; Health aspects; Hydrogen peroxide; hydroxyurea; Kinases; Life Sciences; luciferase; Luciferases - analysis; Luciferases - genetics; maleates; Mammals; menadione; Methods; methyl methanesulfonate; Microbial Genetics and Genomics; Microbiology; Mutagenicity Tests - methods; Mutagens - metabolism; Mutation; oxidants; Oxidants - metabolism; Oxidation; Oxidative Stress; Oxidizing agents; Plasmids; Promoter Regions, Genetic; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Studies; Time Factors; Transcriptional Activation; Yeast; Yeasts; Yeasts (Fungi); GFP; Yeast-based reporter assay; Oxidative damage; Luciferase; DNA damage

We aimed to develop the bioassays for genotixicity and/or oxidative damage using the recombinant yeast. A genotoxicity assay was developed using recombinant Saccharomyces cerevisiae strain BY4741 with a green fluorescent protein (GFP) reporter plasmid, driven by the DNA damage-responsive RNR3 promot...