Development and evaluation of yeast-based GFP and luciferase reporter assays for chemical-induced genotoxicity and oxidative damage - Suzuki, Hajime; Sakabe, Takahiro; Hirose, Yuu; Eki, Toshihiko (Applied microbiology and biotechnology, 2017)
Keywords: Analysis; Applied Genetics and Molecular Biotechnology; Bioassay; Bioassays; Biomedical and Life Sciences; Biotechnology; Cell cycle; Chemicals; Deoxyribonucleic acid; DNA; DNA damage; DNA repair; Fluorescence; Genes; Genes, Reporter; Genetic engineering; Genomes; Genotoxicity; green fluorescent protein; Green Fluorescent Proteins - analysis; Green Fluorescent Proteins - genetics; Health aspects; Hydrogen peroxide; hydroxyurea; Kinases; Life Sciences; luciferase; Luciferases - analysis; Luciferases - genetics; maleates; Mammals; menadione; Methods; methyl methanesulfonate; Microbial Genetics and Genomics; Microbiology; Mutagenicity Tests - methods; Mutagens - metabolism; Mutation; oxidants; Oxidants - metabolism; Oxidation; Oxidative Stress; Oxidizing agents; Plasmids; Promoter Regions, Genetic; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Studies; Time Factors; Transcriptional Activation; Yeast; Yeasts; Yeasts (Fungi); GFP; Yeast-based reporter assay; Oxidative damage; Luciferase; DNA damage